Results 11 to 20 of about 48,352 (276)
PaSS: a sequencing simulator for PacBio sequencing [PDF]
BMC Bioinformatics, 2019 Background Third-generation sequencing platforms, such as PacBio sequencing, have been developed rapidly in recent years. PacBio sequencing generates much longer reads than the second-generation sequencing (or the next generation sequencing, NGS ...
Wenmin Zhang, Ben Jia, Chaochun Wei
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HISEA: HIerarchical SEed Aligner for PacBio data [PDF]
BMC Bioinformatics, 2017 The next generation sequencing (NGS) techniques have been around for over a decade. Many of their fundamental applications rely on the ability to compute good genome assemblies. As the technology evolves, the assembly algorithms and tools have to continuously adjust and improve.
Nilesh Khiste, Lucian Ilie
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“polishCLR: a Nextflow workflow for polishing PacBio CLR genome assemblies” [PDF]
Genome Biology and Evolution, 2022 Abstract Long-read sequencing has revolutionized genome assembly, yielding highly contiguous, chromosome-level contigs. However, assemblies from some third generation long read technologies, such as Pacific Biosciences (PacBio) Continuous Long Reads (CLR), have a high error rate.
Jennifer Chang +5 more
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PACMAN: PacBio Methylation Analyzer
EMBnet.journal, 2015 We developed a tool written in Perl and an associated web site called PACMAN (PacBio Methylation Analyzer) allowing users to easily create images with Circos in a user-friendly interface. From the genome in FASTA format and the motifs in GFF format, PACMAN generates a publication quality image of the selected methylated motifs counts, locations and non-
Laurent Falquet, Alexis Loetscher
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Bi-Level Error Correction for PacBio Long Reads [PDF]
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2020 The latest sequencing technologies such as the Pacific Biosciences (PacBio) and Oxford Nanopore machines can generate long reads at the length of thousands of nucleic bases which is much longer than the reads at the length of hundreds generated by Illumina machines. However, these long reads are prone to much higher error rates, for example 15%, making
Yuansheng Liu +3 more
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RNA Biology, 2022 Endothelial cells (ECs) comprise the lumenal lining of all blood vessels and are critical for the functioning of the cardiovascular system. Their phenotypes can be modulated by alternative splicing of RNA to produce distinct protein isoforms.
Madison M. Mehlferber +9 more
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Single-molecule real-time sequencing combined with optical mapping yields completely finished fungal genome [PDF]
, 2015 Next-generation sequencing (NGS) technologies have increased the scalability, speed, and resolution of genomic sequencing and, thus, have revolutionized genomic studies.
Datema, Erwin +6 more
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Circlator: automated circularization of genome assemblies using long sequencing reads [PDF]
, 2015 The assembly of DNA sequence data is undergoing a renaissance thanks to emerging technologies capable of producing reads tens of kilobases long.
Harris, Simon R. +5 more
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The Gossypium longicalyx Genome as a Resource for Cotton Breeding and Evolution
G3: Genes, Genomes, Genetics, 2020 Cotton is an important crop that has made significant gains in production over the last century. Emerging pests such as the reniform nematode have threatened cotton production.
Corrinne E. Grover +11 more
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Whole-genome sequencing of Klebsiella pneumoniae isolates to track strain progression in a single patient with recurrent urinary tract infection [PDF]
, 2019 Klebsiella pneumoniae is an important uropathogen that increasingly harbors broad-spectrum antibiotic resistance determinants. Evidence suggests that some same-strain recurrences in women with frequent urinary tract infections (UTIs) may emanate from a ...
Minx, Patrick J +3 more
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