Results 41 to 50 of about 48,352 (276)

IPA assembly for PacBio Hifi reads v1

open access: yes, 2021
This protocol is regarding the assembly of plant genomes using HiFi data (PacBio Sequel II) with Improved Phase Assembly (IPA) tool.
Priyanka Sharma   +11 more
openaire   +1 more source

Pacbio Sequencing of PLC/PRF/5 Cell Line and Clearance of HBV Integration Through CRISPR/Cas-9 System

open access: yesFrontiers in Molecular Biosciences, 2021
The integration of HBV DNA is one of the carcinogenic mechanisms of HBV. The clearance of HBV integration in hepatocyte is of great significance to cure chronic HBV infection and thereby prevent the occurrence of HBV-related hepatocellular carcinoma (HCC)
Chia-Chen Chen   +8 more
doaj   +1 more source

Jabba: hybrid error correction for long sequencing reads using maximal exact matches [PDF]

open access: yes, 2015
Third generation sequencing platforms produce longer reads with higher error rates than second generation sequencing technologies. While the improved read length can provide useful information for downstream analysis, underlying algorithms are challenged
Audenaert, P.   +4 more
core   +1 more source

The complete mitochondrial genome of the Antarctic marbled rockcod, Notothenia rossii (Perciformes, Nototheniidae)

open access: yesMitochondrial DNA. Part B. Resources, 2020
The complete mitochondrial genome of Notothenia rossii was obtained using PacBio Sequel long-read sequencing platform. The mitogenome of N. rossii was circular form and 18,274 bp long, which consists of 13 protein-coding genes, 24 tRNAs, 2 rRNAs, and non-
Euna Jo   +7 more
doaj   +1 more source

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

open access: yesMicroorganisms, 2020
Whole-genome DNA sequencing of Lactobacillus D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both
Vyacheslav Toropov   +4 more
doaj   +1 more source

Comparative genome analysis of Wolbachia strain wAu [PDF]

open access: yes, 2014
BACKGROUND: Wolbachia intracellular bacteria can manipulate the reproduction of their arthropod hosts, including inducing sterility between populations known as cytoplasmic incompatibility (CI). Certain strains have been identified that are unable to
Harris, Simon R.   +3 more
core   +2 more sources

Assembly of the durian chloroplast genome using long PacBio reads [PDF]

open access: yesScientific Reports, 2020
AbstractWe have assembled the complete sequence of the Durio zibethinus chloroplast genome using long PacBio reads. Durian is a valuable commercial tree that produces durian fruit, which is popular in Southeast Asia. The chloroplast genome assembled into a single 143 kb cyclic contig that contained 111 genes.
Jeremy R. Shearman   +9 more
openaire   +2 more sources

Complete Genome Sequence of the Citrobacter freundii Type Strain. [PDF]

open access: yes, 2020
Citrobacter freundii is a species of facultative anaerobic Gram-negative bacteria of the family Enterobacteriaceae The complete genome is composed of a single chromosomal circle of 4,957,773 bp with a G+C content of 52%
Booth, Benjamin W   +5 more
core  

PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats

open access: yesScientific Reports, 2023
Abstract The phylum Apicomplexacomprises a large group of protozoan organisms that are obligatory intracellular parasites and can infect various vertebrate and invertebrate hosts, leading to several significant medical and veterinary pathologies, including toxoplasmosis, cryptosporidiosis, theileriosis and eimeriosis.
Mahmoud Gad   +5 more
openaire   +3 more sources

Telomere‐to‐Telomere Genomes Reveal that Multiscale Evolution Shapes the Largest Metabolic Arsenal of Diaporthe Fungi

open access: yesAdvanced Science, EarlyView.
This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li   +9 more
wiley   +1 more source

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