Results 31 to 40 of about 574,806 (288)
Data in Brief, 2020 The complete genome of the thraustochytrid Aurantiochytrium limacinum strain CCAP_4062/1 was sequenced using both Illumina Novaseq 6000 and third generation sequencing technology PacBio RSII in order to obtain trustworthy assembly and annotation.
Christian Morabito +4 more
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Next-Generation Sequencing:Application in Liver Cancer-Past, Present and Future? [PDF]
, 2012 Hepatocellular Carcinoma (HCC) is the third most deadly malignancy worldwide characterized by phenotypic and molecular heterogeneity. In the past two decades, advances in genomic analyses have formed a comprehensive understanding of different underlying ...
Daly +19 more
core +2 more sources
PaSS: a sequencing simulator for PacBio sequencing
BMC Bioinformatics, 2019 Background Third-generation sequencing platforms, such as PacBio sequencing, have been developed rapidly in recent years. PacBio sequencing generates much longer reads than the second-generation sequencing (or the next generation sequencing, NGS ...
Wenmin Zhang, Ben Jia, Chaochun Wei
doaj +1 more source
Challenges in Third-Generation DNA Sequencing [PDF]
Journal of Nanomedicine & Nanotechnology, 2012 DNA sequencing is one of the leading precursors of the personalized medicine, i.e. for reading hereditary traits for predisposition to diseases which are coded in DNA, and thus to prevent, diagnose, and treat diseases. After the success of the Human Genome Project in 2003, which achieved reading a human genome for $3B spent over thirteen years, the ...
openaire +1 more source
A Path to Implement Precision Child Health Cardiovascular Medicine. [PDF]
, 2017 Congenital heart defects (CHDs) affect approximately 1% of live births and are a major source of childhood morbidity and mortality even in countries with advanced healthcare systems. Along with phenotypic heterogeneity, the underlying etiology of CHDs is
Brian Reemtsen +8 more
core +2 more sources
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. [PDF]
, 2017 Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase ...
Bartlett, Anna +12 more
core +2 more sources
Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing
G3: Genes, Genomes, Genetics, 2018 The Drosophila genus is a unique group containing a wide range of species that occupy diverse ecosystems. In addition to the most widely studied species, Drosophila melanogaster, many other members in this genus also possess a well-developed set of ...
Danny E. Miller +3 more
doaj +1 more source
A new targeted CFTR mutation panel based on next-generation sequencing technology [PDF]
, 2017 Searching for mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR) is a key step in the diagnosis of and neonatal and carrier screening for cystic fibrosis (CF), and it has implications for prognosis and personalized therapy ...
Alberti, Luisella +10 more
core +2 more sources
New technologies accelerate the exploration of non-coding RNAs in horticultural plants. [PDF]
, 2017 Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small ...
Hu, Rongbin +4 more
core +3 more sources
An Overview of the Second and Third-Generation of DNA Sequencing Technologies [PDF]
Journal of Advanced Biomedical Sciences, 2017 DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid.
Fatemeh Ghorbani Parsa +1 more
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