Advances in Metagenomics and Its Application in Environmental Microorganisms
Frontiers in Microbiology, 2021 Metagenomics is a new approach to study microorganisms obtained from a specific environment by functional gene screening or sequencing analysis.
Lu Zhang +14 more
doaj +1 more source
Computational methods for 16S metabarcoding studies using Nanopore sequencing data
Computational and Structural Biotechnology Journal, 2020 Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA) genes has been an approach widely used in environmental microbiology, particularly since the advent of high-throughput sequencing technologies.
Andres Santos +3 more
doaj +1 more source
Identification of double heterozygous -α4.2Ⅰ/-α4.2Ⅱ using third-generation sequencing
Hematology, 2023 Objective The 4.2 kb deletion (-α4.2/) is a common a+-thalassemia with a carrier rate, followed by the South-East Asian deletion (–SEA) and the 3.7 kb deletion (-α3.7/). There are few reports about 4.2 kb deletion sub-types.
Liang Liang +5 more
doaj +1 more source
Jabba: hybrid error correction for long sequencing reads [PDF]
, 2016 Background: Third generation sequencing platforms produce longer reads with higher error rates than second generation technologies. While the improved read length can provide useful information for downstream analysis, underlying algorithms are ...
Audenaert, P. +6 more
core +3 more sources
Cheap third-generation sequencing [PDF]
Nature Methods, 2009 By covalently attaching cyclodextrin to a hemolysin nanopore, researchers show single-molecule, label-free sequencing at very high accuracy.
openaire +1 more source
Characterization of the human ESC transcriptome by hybrid sequencing [PDF]
, 2013 Although transcriptional and posttranscriptional events are detected in RNA-Seq data from second-generation sequencing, fulllength mRNA isoforms are not captured.
Afshar, Pegah Tootoonchi +10 more
core +2 more sources
Assessing the Gene Content of the Megagenome: Sugar Pine (Pinus lambertiana). [PDF]
, 2016 Sugar pine (Pinus lambertiana Douglas) is within the subgenus Strobus with an estimated genome size of 31 Gbp. Transcriptomic resources are of particular interest in conifers due to the challenges presented in their megagenomes for gene identification ...
Delfino-Mix, Annette +8 more
core +2 more sources
Detection of Genomic Structural Variants from Next-Generation Sequencing Data [PDF]
, 2015 Structural variants are genomic rearrangements larger than 50?bp accounting for around 1% of the variation among human genomes. They impact on phenotypic diversity and play a role in various diseases including neurological/neurocognitive disorders and ...
D\u27Aurizio, Romina
core +1 more source
Analysis of Splicing Regulation by Third-Generation Sequencing
, 2022 In Metazoa, the diversity of transcripts produced by the RNA Polymerase II is generated essentially through post-transcriptional processing of the nascent transcripts. The regulation of exon inclusion by alternative splicing is one of the main sources of this diversity, which leads to the expansion of the proteome.
Allemand, Eric, Ango, Fabrice
openaire +2 more sources
Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus [PDF]
, 2018 The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the analysis of the polyadenylated fraction of the viral transcriptome using both
Balázs, Zsolt +5 more
core +1 more source