Results 61 to 70 of about 22,977 (294)

Pulmonary Delivery of siRNA Anti‐TNFα‐loaded Lipid Nanoparticles for Rapid Recovery in Murine Acute Lung Injury

Advanced Healthcare Materials, EarlyView.
In a murine model of acute lung injury, pulmonary administration of lipid nanoparticles carrying TNFα siRNA enables targeted delivery to immune cells. The study emphasises the importance of optimizing LNP distribution and delivery timing to improve therapeutic outcomes and develop emergency therapies for lung hyperinflammation.
Qinglin Wang, Jihana Achour, Laila Emam, Younes Louaguenouni, Catherine Cailleau, Françoise Mercier‐Nomé, Séverine Domenichini, Claudine Delomenie, Sezen Gul, Juliette Vergnaud, Nicolas Tsapis, Arnaud Mansart, Djillali Annane, Francois Fay, Elias Fattal   +14 more
wiley   +1 more source

Advances and Strategies in Enhancing mRNA Cancer Vaccines

Advanced Materials, EarlyView.
Messenger RNA (mRNA) vaccines offer a powerful approach for cancer immunotherapy, but their clinical impact remains limited by delivery challenges and suboptimal immune activation. This review discusses key biological barriers and design strategies—including structural optimization, immunomodulation, organ targeting delivery, and advanced nanocarriers ...
Miao Zhang, Shengqi Chen, Haijun Hu, Yenhui Ong, Qianqian Ni   +4 more
wiley   +1 more source

Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH [PDF]

, 2001
The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C.
Hägerdal B., J. A. Saraiva, J. C. Oliveira, M. A. Lemos, Pizzocaro F., Van Loey A., Whitaker J. R.   +6 more
core   +2 more sources

Unraveling the Role of MDK‐SDC4 Interaction in Pancreatic Cancer‐Associated New‐Onset Diabetes by Single‐Cell Transcriptomic Analysis

Advanced Science, EarlyView.
Midkine (MDK) is a mediator of the interaction between pancreatic cancer and beta cells. MDK, which originated from pancreatic ductal adenocarcinoma cells, exerted deleterious effects on paraneoplastic beta cells by binding to the SDC4 receptor on the beta cell surface and subsequently downregulating the Ras signaling pathway, thereby impairing insulin
Zengyu Feng, Jianyao Lou, Chuanshuai Lin, Haocheng Yu, Yuheng Tu, Jiali Gong, Xiawei Li, Yulian Wu   +7 more
wiley   +1 more source

Optimization of RNA Extraction from Rat Pancreatic Tissue

Iranian Journal of Medical Sciences, 2014
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any ...
Sanaz Dastgheib, Cambyz Irajie, Raheleh Assaei, Farhad Koohpeima, Pooneh Mokarram   +4 more
doaj  

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source

A Humanized Anti-CD22-Onconase Antibody-Drug Conjugate Mediates Highly Potent Destruction of Targeted Tumor Cells

Journal of Immunology Research, 2015
Antibody-drug conjugates (ADCs) have evolved as a new class of potent cancer therapeutics. We here report on the development of ADCs with specificity for the B-cell lineage specific (surface) antigen CD22 being expressed in the majority of hematological ...
Tobias Weber, Athanasios Mavratzas, Stefan Kiesgen, Stephanie Haase, Benedikt Bötticher, Evelyn Exner, Walter Mier, Ludger Grosse-Hovest, Dirk Jäger, Michaela A. E. Arndt, Jürgen Krauss   +10 more
doaj   +1 more source

Tumor‐Associated Sympathetic Nerves Promote the Progression of Epstein‐Barr Virus‐Positive Diffuse Large B‐Cell Lymphoma

Advanced Science, EarlyView.
Sympathetic nerve innervation promotes the progression of Epstein‐Barr virus‐positive diffuse large B‐cell lymphoma (EBV+ DLBCL) via β2‐adrenergic receptors. The discovery of enhanced nerve infiltration and β2AR expression reveals a novel mechanism and highlights the sympathetic–β2AR axis as a promising target for therapeutic intervention in EBV+ DLBCL.
Silan Huang, Dexin Lei, Linbin Yang, Aiwei Bi, Yanlou Wang, Peng Zhang, Dongyu Zhuang, Honglian Liu, Qingqing Cai, Man Nie, Yi Xia   +10 more
wiley   +1 more source

Extraction of high-quality RNA from mouse pancreatic tumors

MethodsX, 2020
Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases.
Kranthi Kumar Chougoni, Steven R. Grossman   +1 more
doaj  

Highly sensitive nuclease assay based on chemically-modified DNA or RNA [PDF]

, 2014
Nucleolytic enzymes are associated with various diseases, and several methods have been developed for their detection. DNase expression is modulated in such diseases as acute myocardial infarction, transient myocardial ischemia, oral cancer, stomach ...
Sato Shinobu, Takenaka Shigeori
core   +2 more sources