Results 71 to 80 of about 24,475 (262)

Optimization of RNA Extraction from Rat Pancreatic Tissue

Iranian Journal of Medical Sciences, 2014
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any ...
Sanaz Dastgheib, Cambyz Irajie, Raheleh Assaei, Farhad Koohpeima, Pooneh Mokarram   +4 more
doaj  

The KDM6B/SLC10A2 Axis Suppresses MDSCs Recruitment via ERK/AP‐1 Signaling in Colorectal Cancer

Advanced Science, EarlyView.
In wild‐type intestinal epithelial cells, KDM6B demethylates SLC10A2 to promote its expression, after which SLC10A2 suppresses ERK phosphorylation, inhibiting AP‐1 (Fos/Jun) transcription and chemokine production. In KDM6B‐deficient intestinal epithelial cells, SLC10A2 methylation increases, and its expression decreases.
Zhibo Hu, Jing Xun, Bin Liu, Xiaolin Jiang, Yingdi Han, Huichao Yang, Qi Gao, Ruifang Gao, Aimin Zhang, Xueliang Wu, Dapeng Zhang, Dihua Li, Tian Li, Shimin Yang, Ximo Wang, Xiangyang Yu, Qi Zhang   +16 more
wiley   +1 more source

A Humanized Anti-CD22-Onconase Antibody-Drug Conjugate Mediates Highly Potent Destruction of Targeted Tumor Cells

Journal of Immunology Research, 2015
Antibody-drug conjugates (ADCs) have evolved as a new class of potent cancer therapeutics. We here report on the development of ADCs with specificity for the B-cell lineage specific (surface) antigen CD22 being expressed in the majority of hematological ...
Tobias Weber, Athanasios Mavratzas, Stefan Kiesgen, Stephanie Haase, Benedikt Bötticher, Evelyn Exner, Walter Mier, Ludger Grosse-Hovest, Dirk Jäger, Michaela A. E. Arndt, Jürgen Krauss   +10 more
doaj   +1 more source

Highly sensitive nuclease assay based on chemically-modified DNA or RNA [PDF]

, 2014
Nucleolytic enzymes are associated with various diseases, and several methods have been developed for their detection. DNase expression is modulated in such diseases as acute myocardial infarction, transient myocardial ischemia, oral cancer, stomach ...
Sato Shinobu, Takenaka Shigeori
core   +2 more sources

A20 Facilitates Oxaliplatin Sensitivity in Colorectal Cancer Through Monoubiquitylation of IKK‐β

Advanced Science, EarlyView.
This study demonstrates that the expression of A20, a ubiquitin‐editing enzyme, is positively correlated with oxaliplatin sensitivity in colorectal cancer (CRC). Mechanistically, A20 enhances oxaliplatin sensitivity by inhibiting NF‐κB nuclear translocation through monoubiquitination of IKK‐β.
Fan Luo, Ting Yang, Jiaxin Cao, Qun Chen, Zhenhai Lu, Feiteng Lu, Chaozhuo Lin, Zengfei Xia, Yuanzhong Yang, Peng Li, Wenjuan Ma, Min Luo, Rongxin Zhang   +12 more
wiley   +1 more source

Interaction of Human Pancreatic Ribonuclease with Human Ribonuclease Inhibitor [PDF]

Journal of Biological Chemistry, 2001
تتفاعل الريبونوكليزات الثديية بقوة مع مثبط الريبونوكليز داخل الخلايا. الخلايا حقيقية النواة المعرضة للريبونوكليزات الثديية محمية من تأثيرها السام للخلايا عن طريق تثبيط الريبونوكليزات داخل الخلايا بواسطة RI. يتشابه الريبونوكلياز البنكرياسي البشري (HPR) هيكليًا ووظيفيًا جدًا مع الريبونوكلياز أ البقري ويتفاعل مع الريبونوكلياز البشري ذو التقارب العالي.
Deepak Gaur, Srividya Swaminathan, Janendra K. Batra   +2 more
openaire   +1 more source

GlycoChat Uncovers Glycan–Lectin Circuits in the Tumor Microenvironment of Pancreatic Cancer

Advanced Science, EarlyView.
Aberrant glycosylation drives cancer progression, yet its role in the tumor microenvironment remains unclear. We developed GlycoChat to map glycan–lectin circuits at single‐cell resolution. We discovered that cancer cells induce immunosuppressive macrophage differentiation and impair phagocytosis through interactions with CLEC10A and SIGLEC3 ...
Dinh Xuan Tuan Anh, Sunanda Keisham, Arun Burramsetty, Lalhaba Oinam, Koichiro Kumano, Akihiro Kuno, Osamu Shimomura, Tatsuya Oda, Hiroaki Tateno   +8 more
wiley   +1 more source

Dependence of ethanol effects on protein charges [PDF]

, 2014
Ethanol is used as a conventional disinfectant solution. It is highly effective against enveloped viruses due to its effects on virus membranes. It also confers inactivation of non-enveloped viruses, which can be ascribed to conformational changes or ...
Arakawa Tsutomu, Shiraki Kentaro, Yoshizawa Shunsuke, 白木 賢太郎   +3 more
core   +1 more source

IL‐4/STAT6‐signaling Influences Local Inflammation and Regeneration Processes During Acute Pancreatitis and Promotes Fibrosis by a Direct Activation of Pancreatic Fibroblasts During Chronic Pancreatitis

Advanced Science, EarlyView.
IL‐4/IL‐13/STAT6 signaling plays a crucial for the suppression of pro‐inflammation during acute pancreatitis and supports acinar cell regeneration but has only minor impact on fibrogenesis during chronic form of the disease. IL‐4/IL‐13 induce the expression of certain collagens directly in pancreatic fibroblasts via STAT6 activation, whereas ...
ElSheikh H, Salvers A, Piesker J, Buchholz S, Lange R, Ameling S, Steil L, Hammer E, Homuth G, Völker U, Bröker BM, Weiss FU, Sendler M, Glaubitz J   +13 more
wiley   +1 more source

PARN Maintains RNA Stability to Regulate Insulin Maturation and GSIS in Pancreatic β Cells

Advanced Science
Diabetes, a metabolic disorder characterized by hyperglycemia, underscores the importance of normal pancreatic β‐cell development and function in maintaining glucose homeostasis.
Xiaomei Xie, Xuexue Chen, Chaofan Wang, Longjie Sun, Weiru Yu, Zheng Lv, Shuang Tian, Xiaohong Yao, Fengchao Wang, Deqiang Ding, Juan Chen, Jiali Liu   +11 more
doaj   +1 more source