Results 31 to 40 of about 359,301 (251)

Revised paper: Neonicotinoids as a potential threat to honey: Development of liquid chromatography methods for their determination [PDF]

Journal on Processing and Energy in Agriculture, 2017
There is an increasing interest on neonicotinoid residues determination in honey samples because they are banned by European Union. Their determination is based on liquid chromatography analytical methods and, therefore, the aim of this study was to ...
Jovanov Pavle, Sakač Marijana, Šarić Bojana, Milovanović Ivan, Jambrec Dubravka, Šarić Ljubiša, Kos Jovana   +6 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Evaluation of gel chromatography for plasma lipoprotein fractionation

Journal of Lipid Research, 1970
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata, David L. Estrich, Peter D.S. Wood, Laurance W. Kinsell   +3 more
doaj   +1 more source

Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper

Sensing and Bio-Sensing Research, 2020
Detection of mycotoxins by conventional methods such as ELISA or LC-MS can be expensive and time-consuming. Therefore, paper-based biosensors can be effectively used for on-site analysis, due to their low cost and easy detection procedures. Nevertheless,
V.A. Mirón-Mérida, M. Wu, Y.Y. Gong, Y. Guo, M. Holmes, R. Ettelaie, F.M. Goycoolea   +6 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Novel design of low volume mobile phase paper chromatography

Green Analytical Chemistry
A novel design and set up of paper chromatography in the form of radial serpentine arms laying horizontally is proposed. The consumption of mobile phase is reduced to be 1 mL or less as compared to the tens of mL typically used in the conventional set ...
Jacob DeMott, Zachary Hilvano, Hunter Cann, Isa Baldwin-Zurek, William Kershisnik, Supaporn Kradtap Hartwell   +5 more
doaj   +1 more source

Assay of nanogram levels of triglyceride lipase with a radioactive substrate

Journal of Lipid Research, 1972
A simple, sensitive procedure for the determination of triglyceride lipase activity has been developed. Nanogram amounts of oleic acid hydrolyzed from commercially available [14C]triolein were readily determined by the counting of the radioactivity of ...
Walton H. Marsh, Patrick J. Fitzgerald
doaj   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Plant pigments (antioxidants) of medicinal plants Malva Sylvestris l. and Malva Moschata l. (Malvaceae)

Biomolecules & Biomedicine, 2005
Qualitative-quantitative structure of plant pigments in wild plants Malva silvestrs L. and Malva moschata L. (Malvaceae), which were collected in 20 locations in Sarajevo area and surroundings, was tested during spring and summer in 2003.
Sulejman Redžić, Nizama Hodžić, Mijat Tuka   +2 more
doaj   +1 more source