Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Revised paper: Neonicotinoids as a potential threat to honey: Development of liquid chromatography methods for their determination [PDF]
Journal on Processing and Energy in Agriculture, 2017 There is an increasing interest on neonicotinoid residues determination in honey samples because they are banned by European Union. Their determination is based on liquid chromatography analytical methods and, therefore, the aim of this study was to ...
Jovanov Pavle +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Study on Raman and SERS spectra of filter paper and silica gel plate
Journal of Measurement Science and Instrumentation, 2012 Raman spectra and surface-enhanced Raman scattering（SERS）spectra of filter paper and silica gel plate are ob-tained at785-nmexcitation wavelength.The results showthat filter paper has thirteen Ramanlines in800-1 500cm-1and silica gel plate has eight ...
OUYANG Yu
doaj
Evaluation of gel chromatography for plasma lipoprotein fractionation
Journal of Lipid Research, 1970 The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata +3 more
doaj +1 more source
Pyrolysis of brominated feedstock plastic in a fluidised bed reactor [PDF]
, 2006 Fire retarded high impact polystyrene has been pyrolysed using a fluidised bed reactor with a sand bed. The yield and composition of the products have been investigated in relation to fluidised bed temperature.
Bhaskar +10 more
core +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Assay of nanogram levels of triglyceride lipase with a radioactive substrate
Journal of Lipid Research, 1972 A simple, sensitive procedure for the determination of triglyceride lipase activity has been developed. Nanogram amounts of oleic acid hydrolyzed from commercially available [14C]triolein were readily determined by the counting of the radioactivity of ...
Walton H. Marsh, Patrick J. Fitzgerald
doaj +1 more source
Phospholipides containing amino acids other than serine. I. Detection [PDF]
, 1957 In view of the widespread occurrence of the amino acid-containing lipides and the unique course of their metabolism during development of Drosophila, we have carried out extensive investigations concerned with their isolation and chemical nature.
Mitchell, Herschel K. +2 more
core
Response to Tsikas et al. comments on Boelaert et al. Determination of asymmetric and symmetric dimethylarginine in serum from patients with chronic kidney disease: UPLC-MS/MS versus ELISA. Toxins 2016, 8, 149 [PDF]
, 2016 n/
Boelaert, Jente +5 more
core +2 more sources