Results 61 to 70 of about 18,912,087 (403)
Novel design of low volume mobile phase paper chromatography
A novel design and set up of paper chromatography in the form of radial serpentine arms laying horizontally is proposed. The consumption of mobile phase is reduced to be 1 mL or less as compared to the tens of mL typically used in the conventional set ...
Jacob DeMott+5 more
doaj +1 more source
Food, drugs, dyes, extracts, and minerals are all made up of complex elements, and utilizing unidimensional chromatography to separate them is inefficient and insensitive.
Ruhul Amin+6 more
doaj +1 more source
The chromatographic identification of some biologically important phosphate esters [PDF]
the objective of the present work was to provide a means for separating and indentifying phosphate esters involved in glycolysis in higher plants. Paper chromatography of phosphate esters has been employed by several workers, most notably Benson et al ...
Axelrod, Bernard, Bandurski, Robert S.
core
Influence of the sebaceous gland density on the stratum corneum lipidome [PDF]
The skin surface lipids (SSL) result from the blending of sebaceous and epidermal lipids, which derive from the sebaceous gland (SG) secretion and the permeability barrier of the stratum corneum (SC), respectively.
Camera, Emanuela+5 more
core +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Evaluation of gel chromatography for plasma lipoprotein fractionation
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata+3 more
doaj +1 more source
Obtaining and Characteristic of the Autolysate of Lactic Acid Bacteria [PDF]
Fragments of bacterial peptidoglycan relating to compounds of the muropeptide series have high biological activity. They have an immunological effect, due to the fact that they are signals for immune receptors and, accordingly, their activators. In order
Cherno, N. (Natalia)+1 more
core +2 more sources
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Assay of nanogram levels of triglyceride lipase with a radioactive substrate
A simple, sensitive procedure for the determination of triglyceride lipase activity has been developed. Nanogram amounts of oleic acid hydrolyzed from commercially available [14C]triolein were readily determined by the counting of the radioactivity of ...
Walton H. Marsh, Patrick J. Fitzgerald
doaj +1 more source
Silylation of Carbohydrate Syrups [PDF]
Introduction Carbohydrates are usually difficult to analyze in solutions. Gas chromatography provides a suitable means for analysis. However most carbohydrate compounds are not volatile enough for use by this method.
Gorwitz, Mark
core +1 more source