Results 11 to 20 of about 521,053 (283)

Quantitation of serum lipoproteins by electrophoresis on agarose gel: standardization in lipoprotein concentration units (mg/100 ml) by comparison with analytical ultracentrifugation

open access: yesJournal of Lipid Research, 1971
Lipoprotein electrophoresis on agarose gel has been modified to allow estimation of the absolute quantity of each fraction. The reproducibility of the method is illustrated by 12 determinations in a single day on serum from one normal subject: mean total
S.B. HULLEY   +5 more
doaj   +1 more source

Electrophoretic Separation on an Origami Paper-Based Analytical Device Using a Portable Power Bank

open access: yesSensors, 2019
The electrophoresis of ampholytes such as amino acids on a paper device is difficult because of the variation of pH distribution in time. On the basis of this observation, we propose a paper-based analytical device (PAD) with origami structure.
Yu Matsuda   +3 more
doaj   +1 more source

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

open access: yesProteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus   +2 more
doaj   +1 more source

Flash-comet assay

open access: yesMethodsX, 2020
In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj   +1 more source

Results of conducting the primary seed production using the method of electrophoresis in the FSBSI “ARC “Donskoy

open access: yesЗерновое хозяйство России, 2022
The current paper has presented the study and estimation results of winter bread wheat varieties in primary seed production (2019-2021) based on the method of electrophoresis.
T. I. Firsova   +3 more
doaj   +1 more source

Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]

open access: yes, 1978
The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François   +3 more
core   +1 more source

Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis

open access: yesJournal of Lipid Research, 1973
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described.
Neil Kaplowitz, Norman B. Javitt
doaj   +1 more source

A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection [PDF]

open access: yesRoyal Society Open Science, 2018
A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper.
Mingpeng Yang, Zhe Huang, Hui You
doaj   +1 more source

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

open access: yes, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

open access: yesClinical Proteomics, 2011
Introduction The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis.
Seshi Beerelli   +2 more
doaj   +1 more source

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