Results 21 to 30 of about 521,053 (283)

Western blotting of native proteins from agarose gels

BioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta   +10 more
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold, An, Andersen, Anderson, Anderson, Anderson, Anderson, Anderson, Appel, Artoni, Baczek, Baczek, Bell, Berggren, Bjellqvist, Bjellqvist, Bonner, Braun, Bravo, Bravo, Bravo, Bravo, Brown, Bunai, Bunger, Burre, Bury, Caglio, Caglio, Camacho-Carvajal, Cantin, Cargile, Cargile, Cargile, Castellanos-Serra, Cejka, Chen, Chevalier, Chevallet, Chiari, Chiari, Chick, Claverol, Cleveland, Cohen, Colinge, Corbett, Coughenour, Courchesne, Cox, Daban, Daban, Davis, Domon, Dukan, Duncan, Egen, Eng, Eravci, Essader, Fernandez-Patron, Figeys, Fritz, Garrels, Garrels, Gelfi, Gelfi, Giometti, Gordon, Gordon, Gorg, Gorg, Gorg, Groleau, Gronow, Han, Hardison, Hartinger, Heller, Henrich, Henzel, Hisabori, Hoffert, Hoffmann, Horowitz, Horth, Hunzinger, James, Johnson, Johnston, Jorgensen, Jovin, Jovin, Kasicka, Keller, Kobayashi, Krijgsveld, Krokhin, Kuroda, Kuster, Kyte, Laemmli, Lambert, Langen, Lasonder, Lengqvist, Liang, Link, Liu, Locke, Loo, Lopez, Lu, Lu, Lu, Luche, Lui, Luo, Luque-Garcia, Lyubimova, Macfarlane, Macfarlane, MacGillivray, Mackintosh, Malmstrom, Malone, Matsudaira, Mazzeo, Mets, Moritz, Nakamura, Navarre, Neuhoff, Nielsen, Novakova, O'Farrell, O'Farrell, Ogorzalek Loo, Orrick, Ortiz, Oses-Prieto, Pappin, Patton, Peng, Perkins, Petritis, Phelps, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Radola, Radola, Rais, Reid, Reynolds, Richert, Rigaut, Righetti, Righetti, Righetti, Righetti, Righetti, Ros, Rosenfeld, Sanchez, Santoni, Schagger, Schagger, Scherl, Schirle, Schluesener, Schmitt, Sevinsky, Sevinsky, Shang, Shapiro, Shen, Shimura, Shimura, Siemankowski, SimoAlfonso, Simpson, Steinberg, Steinberg, Switzer, Szponarski, Szponarski, Tabb, Tan, Tarroux, Tastet, Towbin, Tsugita, Tuszynski, Unlu, van Oostveen, Vonguyen, Voris, Wang, Wang, Washburn, Weber, Wen, West, White, Wilkins, Williams, Wiltfang, Winkler, Wise, Witze, Xiao, Xie, Xie, Yamaguchi, Yamaguchi, Yata, Yates, Young, Yu, Yuan, Zahedi, Zewert, Zischka, Zuo   +225 more
core   +4 more sources

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

Micromachines, 2021
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez, Francisco Perdigones, José Manuel Quero   +2 more
doaj   +1 more source

Integration of biological data resources using image object keying. [PDF]

, 2003
This paper proposes a novel concept of ‘image object keying'. The work builds on earlier research in this area and shows how the 3D structure of a protein can be retrieved interactively from a gel electrophoresis protein spot.
Khan, Nawaz, Rahman, Shahedur, Stockman, A. G.   +2 more
core   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

µTransparent insulating channels as components for miniaturized chemical separation Devices [PDF]

, 2000
Currently, miniaturized devices that apply electro osmotic pumping or electrophoretic separations are mostly constructed by etching small insulating channels for supply and separation on glass substrates.
Berg, A. van den, Hendrikse, J., Schasfoort, R.B.M.   +2 more
core   +2 more sources

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

The design of mechatronic system for gel electrophoresis [PDF]

MATEC Web of Conferences, 2020
The paper present the methodology to design and implement a mechatronic system for gel electrophoresis. It describes the steps towards the development of a prototype and presents its functions. The new automated system is characterized by high precision,
Tsagaris Apostolos, Theodoridis Konstantinos, Stergiopoulos Fotis, Bechtsis Dimitrios, Nikolaidis Nikolaos, Triantafyllidis Dimitrios, Papaoikonomou Asterios, Filelis Anastasios   +7 more
doaj   +1 more source

20 Years of Fatty Acid Analysis by Capillary Electrophoresis

Molecules, 2014
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira, Brenda Lee Simas Porto, Isaura Daniele Leite Faria, Patricia Lopes de Oliveira, Patricia Mendonça de Castro Barra, Renata de Jesus Coelho Castro, Renata Takabayashi Sato   +6 more
doaj   +1 more source

Short-Chained Oligo(Ethylene Oxide)-Functionalized Gold Nanoparticles: Realization Of Significant Protein Resistance [PDF]

, 2017
Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to ...
Riley, Kathryn R., Sims, C. M., Vanderah, D. J., Walker, M. L., Wood, I. T.   +4 more
core   +2 more sources