Immunofluorescence Staining of Paraffin Sections Step by Step [PDF]
Frontiers in Neuroanatomy, 2020 Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers.
Sami Zaqout +6 more
doaj +3 more sources
A One-Stop Protocol to Assess Myocardial Fibrosis in Frozen and Paraffin Sections [PDF]
Methods and Protocols, 2022 Masson’s Trichrome Staining (MTS) is a useful tool for analyzing fibrosis in a plethora of disease pathologies by differential staining of tissue components. It is used to identify collagen fibers in different tissues like heart, lung, skin, and muscles.
Divya Sridharan +5 more
doaj +3 more sources
Pancreatic Islet Embedding for Paraffin Sections. [PDF]
J Vis Exp, 2018 Experiments using isolated pancreatic islets are important for diabetes research, but islets are expensive and of limited abundance. Islets contain a mixed cell population in a structured architecture that impacts function, and human islets are widely variable in cell type composition.
Kong Y +4 more
europepmc +5 more sources
Enhanced Histochemical Detection of Iron in Paraffin Sections of Mouse Central Nervous System Tissue [PDF]
ASN Neuro, 2016 Histochemical methods of detecting iron in the rodent brain result mainly in the labeling of oligodendrocytes, but as all cells utilize iron, this observation suggests that much of the iron in the central nervous system goes undetected.
Scott A. Sands +4 more
doaj +3 more sources
Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer [PDF]
Diagnostic Pathology, 2023 Background PD-L1 staining using long-stored paraffin sections may not be consistent with the true PD-L1 expression of patients. Therefore, it is necessary to explore the expression of PD-L1(SP142) in paraffin sections of invasive breast cancer with ...
Jiankun He +9 more
doaj +2 more sources
Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy [PDF]
Scientific Reports, 2021 Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested.
Aiste Vitkunaite +6 more
doaj +2 more sources
A Simplified Method for the Histochemical Detection of Iron in Paraffin Sections: Intracellular Iron Deposits in Central Nervous System Tissue [PDF]
ASN Neuro, 2021 Although all cells contain iron, most histochemical methods fail to reveal the presence of iron within many cells of the central nervous system (CNS), particularly neurons.
Steven M. LeVine +2 more
doaj +2 more sources
Diagnosis of Ion-Exchange Resin Depositions in Paraffin Sections Using Corrective Light and Electron Microscopy-NanoSuit Method [PDF]
Diagnostics, 2021 Ion-exchange resins are commonly used to treat complications such as hyperkalemia, hyperphosphatemia, and hypercholesterolemia. Gastrointestinal complications may occur as side effects of such treatments.
Mako Ooishi +10 more
doaj +2 more sources
Frozen sections compared with paraffin-embedded sections: A retrospective veterinary autopsy study
Brazilian Journal of Veterinary Pathology, 2020 Histologic examination of tissues collected at autopsy are typically prepared using conventional methods (formalin fixed, paraffin‐embedded tissue). In this study, twenty-five tissue specimens from twelve animals were collected at autopsy and examined ...
Adam W. Stern
doaj +2 more sources
Paraffin sections may be useful in Mohs micrographic surgery for nonmelanoma skin cancer in select patients [PDF]
JAAD InternationalRyan T. Ladd, MD +5 more
doaj +2 more sources