Results 21 to 30 of about 3,658,490 (342)

Reliable PCR quantitation of estrogen, progesterone and ERBB2 receptor mRNA from formalin-fixed, paraffin-embedded tissue is independent of prior macro-dissection [PDF]

, 2013
Gene expression analysis on messenger RNA (mRNA) purified from formalin-fixed, paraffin-embedded tissue is increasingly used for research purposes. Tissue heterogeneity may question specificity and interpretation of results from mRNA isolated from a ...
Alsner, Jan, Hennig, Guido, Kyndi, Marianne, Myhre, Simen, Overgaard, Jens, Sørensen, Flemming Brandt, Sørlie, Therese, Tramm, Trine   +7 more
core   +1 more source

Comparison of Different Methodologies and Cryostat versus Paraffin Sections for Chromogenic Immunohistochemistry

Acta Histochemica, 2019
Immunohistochemistry (IHC) specifically localizes proteins in cells and tissues, but methodologies vary widely. Therefore, we performed a methodological IHC optimization and validation study.
Vashendriya V. V. Hira, Annique Loncq de Jong, Klea Ferro, M. Khurshed, Remco J. Molenaar, C. van Noorden   +5 more
semanticscholar   +1 more source

Comparative Physiological Analysis of Lignification, Anthocyanin Metabolism and Correlated Gene Expression in Red Toona sinensis Buds during Cold Storage

Agronomy, 2022
The characteristics of anthocyanin and lignin are important parameters in evaluating the quality of red Toona sinensis buds. Red T. sinensis buds are prone to senescence during postharvest storage, which subsequently affects their quality and sales ...
Qian Zhao, Xiu-Lai Zhong, Xia Cai, Shun-Hua Zhu, Ping-Hong Meng, Jian Zhang, Guo-Fei Tan   +6 more
doaj   +1 more source

A novel immunohistochemical protocol for paraffin embedded tissue sections using free-floating techniques

Frontiers in Neuroanatomy, 2023
Immunohistochemistry (IHC) is a well-established and widely used protocol used to visualize tissue architecture, protein expression and localization. Free-floating methods for IHC employ tissue sections that are cut from a cryostat or vibratome.
Carolina Muniz Partida, Eric Walters
doaj   +1 more source

The value of frozen section diagnosis of tumor spread through air spaces in small-sized (≤ 2 cm) non-small cell lung cancer

World Journal of Surgical Oncology, 2023
Background The current accuracy of frozen section diagnosis of tumor spread through air spaces (STAS) in non-small cell lung cancer (NSCLC) is poor. However, the accuracy and prognostic value of STAS assessment on frozen sections in small-sized NSCLC ...
Yun Ding, Shutong Zhao, Xin Liu, Jie Ren, Jiuzhen Li, Weiran Zhang, Meilin Xu, Daqiang Sun   +7 more
doaj   +1 more source

From fix to fit into the autoptic human brains. [PDF]

, 2018
Formalin-fixed, paraffinembedded (FFPE) human brain tissues are very often stored in formalin for long time. Formalin fixation reduces immunostaining, and the DNA/RNA extraction from FFPE brain tissue becomes suboptimal.
Lavezzi, A., Paradiso, B, Simonato, M
core   +1 more source

A STUDY OF AREA AND THICKNESS COMPRESSION OF PARAFFIN SECTIONS

Image Analysis and Stereology, 2018
This study was carefully designed to determine the section compression of paraffin embedded sections. Two sections (one with thickness 5 µm and one 10 µm set by microtome) were cut from each of 2 sets of 12 testicular tissue (adult rats) blocks and ...
Yu Xiang, Yang Guo, Zheng-Wei Yang
doaj   +1 more source

Improving automated thyroid cancer classification of frozen sections by the aid of virtual image translation and stain normalization

Computer Methods and Programs in Biomedicine Update, 2023
Frozen sections are rapidly generated during surgical interventions. This allows surgeons to wait for histological findings during the interventions in order to base intra-surgical decisions on the outcome of the histology.
Michael Gadermayr, Maximilian Tschuchnig, Lea Maria Stangassinger, Christina Kreutzer, Sebastien Couillard-Despres, Gertie Janneke Oostingh, Anton Hittmair   +6 more
doaj   +1 more source

Técnica de peroxidase-antiperoxidase (PAP) em biópsia renal: estudo comparativo

Memorias do Instituto Oswaldo Cruz, 1980
Os métodos de imunoperoxidase têm muito em comum com os da imunofluorescência para a demonstração de antígenos teciduais e celulares como no campo das doenças renais relacionadas a imunoglobinas e imunocomplexos. Neste trabalho os autores fazem um estudo
Euzenir Nunes Sarno, Leila Maria Machado Vieira, Frederico Ruzany, Celia Szwarcwald, Jayme Landmann   +4 more
doaj   +1 more source

Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function

Frontiers in Ecology and Evolution, 2023
IntroductionThe longitudinal section cell morphology of Chinese fir roots was studied to better understand the relationship between root structure and root function.MaterialsIn this study, the adjusted microwave paraffin section method and the selected ...
Linxin Li, Linxin Li, Ting Zhang, Ting Zhang, Jingquan Lin, Xiaoqian Lian, Xiaoqian Lian, Xianhua Zou, Xiangqing Ma, Xiangqing Ma, Pengfei Wu, Pengfei Wu   +11 more
doaj   +1 more source