The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy. [PDF]
Lab Invest, 2020 Histological examination using the light microscopy is currently the gold standard for life science research and diagnostics. However, magnified observations are limited because of the limitations intrinsic to light microscopy.
Kawasaki H +7 more
europepmc +2 more sources
Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy. [PDF]
Sci Rep, 2018 Recent advances in bio-medical research, such as the production of regenerative organs from stem cells, require three-dimensional analysis of cell/tissue architectures.
Sawaguchi A +6 more
europepmc +2 more sources
On the acceptance of “fake” histopathology: A study on frozen sections optimized with deep learning
Journal of Pathology Informatics, 2022 Background: The fast acquisition process of frozen sections allows surgeons to wait for histological findings during the interventions to base intrasurgical decisions on the outcome of the histology.
Mario Siller +11 more
doaj +3 more sources
Düzce Tıp Fakültesi Dergisi, 2023 Aim: This study aimed to compare several specific staining protocols recommended for paraffin sections and toluidine blue and light green double staining combination to be tried for the first time with routine toluidine blue staining on semithin epon ...
Ayşe Akbaş +3 more
doaj +1 more source
Frontiers in Physics, 2023 Objective: To establish a rapid and effective method for en-bloc hematoxylin-eosin (HE) staining and paraffin embedding of human lung cancer and paracancerous tissues which can be applied to fluorescence micro-optical sectioning tomography (fMOST ...
Yun Ding +7 more
doaj +1 more source
Immunofluorescence on Formalin Fixed Paraffin Embedded Renal Tissue Sections: A Retrospective Study [PDF]
Journal of Clinical and Diagnostic Research, 2022 Introduction: Direct Immunofluorescence (DIF)/Routine Immunofluorescence (R-IF) on frozen sections is vital in the work-up of renal diseases. Sometimes, the unfixed sample may not be available for DIF or the sample may be inadequate.
Renuka Malipatel +2 more
doaj +1 more source
Quantitative profiling of the human substantia nigra proteome from laser-capture microdissected FFPE tissue [PDF]
, 2020 Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens.
Griesser, Eva +5 more
core +3 more sources
ADAM10 Localization in Temporomandibular Joint Disk with Internal Derangement: An Ex Vivo Immunohistochemical Study [PDF]
, 2016 The purpose of this study was to determine the presence of ADAM10 in temporomandibular joint disk with internal derangement. Twenty-five paraffin blocks of displaced temporomandibular joint (TMJ) disk specimens from earlier investigations were retrieved ...
Almeida, Luis Eduardo +7 more
core +2 more sources
Acta Neuropathologica, 2006 Assessment of Alzheimer’s disease (AD)-related neurofibrillary pathology requires a procedure that permits a sufficient differentiation between initial, intermediate, and late stages.
H. Braak +4 more
semanticscholar +1 more source
Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification [PDF]
, 1993 We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and
Cremer, Thomas +7 more
core +1 more source