Results 61 to 70 of about 891,924 (262)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Differences in dissolved organic matter and analysis of influencing factors between plantations pure and mixed forest soils in the loess plateau

Frontiers in Forests and Global Change
The dissolved organic matter (DOM) in forest ecosystems significantly impacts soil carbon cycling due to its active turnover characteristics. However, whether different plantation forest soil profiles exhibit distinct DOM characteristics remains unclear.
Yongxia Meng, Peng Li, Lie Xiao, Bingze Hu, Chaoya Zhang, Shutong Yang, Jialiang Liu, Binhua Zhao   +7 more
doaj   +1 more source

Styles of providing negative information by doctors on the basis of the analysis of the Breaking Bad News Skills questionnaire

Psychiatria i Psychologia Kliniczna, 2018
Aim of the study: Identification of factors which determine effective communication between a doctor and a patient. Development of a tool for the evaluation of doctors’ skills of breaking bad news to patients’ relatives. Material and methods: The study
Jakub Lickiewicz, Wioletta Szwed-Łopata, Kinga Sałapa, Marta Makara-Studzińska   +3 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Comparison of factor retention methods on binary data: A simulation study

Turkish Journal of Education, 2019
In this study, the purpose is to compare factor retention methods under simulation conditions. For this purpose, simulations conditions with a number of factors (1, 2 [simple]), sample sizes (250, 1.000, and 3.000), number of items (20, 30), average ...
Abdullah Faruk Kılıç, İbrahim Uysal
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Characteristics of Dissolved Organic Carbon Components and Their Responses to Carbon Degradation Genes in Black Soil Under Long-Term Fertilization

Agronomy
Dissolved organic carbon (DOC) represents the most readily available and crucial carbon source for soil microorganisms, influencing their community structure, nutrient cycling, and metabolic functions.
Xiaoyu Han, Wenyan Shen, Enjiang Xiong, Hongfang Liu, Renlian Zhang, Zhimei Sun, Shuxiang Zhang   +6 more
doaj   +1 more source