Results 111 to 120 of about 167,710 (314)

Carbon Dioxide (CO2) Emissions in Latin America: Looking for the Existence of Environmental Kuznets Curves

We estimated environmental Kuznets curve (EKC) for carbon dioxide for 16 Latin American countries using nonparametric, semi-parametric, and parametric specifications.
Zapata, Hector O., Diaz, Alehandro, Bhattarai, Keshav, Paudel, Krishna P.   +3 more
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Kuznets curve and environmental performance: evidence from China

The paper investigates China’s environmental performance-economic development relationship for the time period of 1965-2009. The results indicate that after 1990 China increased its environmental performance mainly driven by the implementation of several
Halkos, George, Tzeremes, Nickolaos
core  

Efficient Yield Curve Estimation and Forecasting in Brazil [PDF]

Term Structure of the Interest Rate, Yield Curve, State-Space Model, Kalman Filter.
Guilherme V. Moura, Marcelo S. Portugal, Ricardo Azevedo Araujo   +2 more
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Evaluation Algorithms for Parametric Curves and Surfaces

Mathematics
This paper extends Woźny and Chudy’s linear-complexity Bézier evaluation algorithm (2020) to all parametric curves/surfaces with normalized basis functions via a novel basis function matrix decomposition. The unified framework covers the following: (i) B-
Lanlan Yan
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Non Parametric Confidence Intervals for Receiver Operating Characteristic Curves [PDF]

We study methods for constructing confidence intervals, and confidence bands, for estimators of receiver operating characteristics. Particular emphasis is placed on the way in which smoothing should be implemented, when estimating either the ...
Rob J. Hyndman, Yanan Fan, Peter G. Hall
core