Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Testing the altruism hypothesis with italian cohort data
In this paper I follow Abel and Kotlikoff 1994 non-parametric approach based on consumption cohort data to test for intergenerational altruism among Italian households. The Italian socio-economic framework represents an interesting ground to test for the
Rizza, Pietro
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Economies of Scale and Hospital Productivity: An empirical analysis of medical area level panel data [PDF]
This paper estimates the total factor productivity (TFP) of hospitals by using panel data drawn from prefectures and secondary medical areas. The study focuses on the economies of scale at the medical area and hospital levels.
MORIKAWA Masayuki
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Comment on "Do we need flexible machine-learning algorithms to assess the effect of long-term exposure to fine particulate matter on mortality?" [PDF]
Williams N, Rudolph KE, Díaz I.
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Familiarity, attitude and practice of postgraduate health science students of Pakistan regarding the implication of artificial intelligence in research: an analytical survey. [PDF]
Rahim N +9 more
europepmc +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
The Impact of Complex Oral Rehabilitation on TMJ and Postural Alterations in Patients with Scapulohumeral Fractures. [PDF]
Stamatin O +11 more
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
The effects of governance changes on bank efficiency in China: A stochastic distance function approach [PDF]
China has accelerated and deepened bank reform since it joined the WTO in 2001. Employing a stochastic distance function, this paper investigates the technical efficiency of banks and examines the static, selection and dynamic effects of governance ...
Chunxia Jiang, Shujie Yao
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