Results 71 to 80 of about 155,370 (254)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

The effect of parental school involvement on student academic achievement in Somalia: moderating role of parental education

open access: yesDiscover Education
The study aimed to examine the effect of parental school involvement on student academic achievement in Somalia, with a focus on the moderating role of parental education.
Yonis Abdullahi Hirei   +6 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

open access: yesFEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão   +3 more
wiley   +1 more source

Parental Involvement in the Schooling of Children with Special Educational Needs

open access: yesKülönleges Bánásmód
This study explores the association between special educational needs (SEN) and the level, mode, and intensity of parental involvement in Hungarian schools.
Katinka Bacskai, Anikó Varga Nagy
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Parent Satisfaction : Contribution of Parental Involvement and School Service Quality Perception at Public Elementary School

open access: yesJurnal Paedagogy
This study aims to identify the characteristics, parental involvement, school service quality, and parent satisfaction of public elementary school students and analyze the influence of parental involvement and school service quality on parent ...
Anindya Savira   +2 more
doaj   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

open access: yesFEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell   +8 more
wiley   +1 more source

How parental involvement in youth sports impacts on school adjustment: a dual mediation pathway via sports interest and extracurricular sports participation

open access: yesFrontiers in Public Health
IntroductionThis study aimed to examine whether parental involvement in youth sports influences Chinese adolescents’ school adjustment and to investigate the mediating roles of extracurricular sports participation and sports interest in this relationship.
Haoyuan Zheng, Zubing Xiang, Wenli Yang
doaj   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

open access: yesFEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson   +2 more
wiley   +1 more source

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