Results 101 to 110 of about 3,658,729 (281)
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
The BEM for numerical solution of partial fractional differential equations
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We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The aim of this paper is to present an efficient and reliable treatment of the Homotopy perturbation method (HPM) and the modified Riemann –Liouville derivative for partial differential equations with fractional time derivative.
Abeer Majeed
doaj
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
The Jacobi Elliptic Equation Method for Solving Fractional Partial Differential Equations
Based on a nonlinear fractional complex transformation, the Jacobi elliptic equation method is extended to seek exact solutions for fractional partial differential equations in the sense of the modified Riemann-Liouville derivative. For demonstrating the validity of this method, we apply it to solve the space fractional coupled Konopelchenko-Dubrovsky (
Bin Zheng, Qinghua Feng
openaire +4 more sources
Exact solutions for a class of space-time fractional mixed (1+1)-dimensional KdV equations
Exact solutions of a class of space-time fractional mixed (1+1)-dimensional KdV equations with modified Riemann-Liouville fractional derivative are considered.
LI Lin-Fang, SHU Ji, WEN Hui-Xia
doaj
This study examines approximate analytical solutions of the time-fractional Swift–Hohenberg equation involving the Caputo–Fabrizio fractional derivative.
Mustafa Ahmed Ali, Mehmet Merdan
doaj +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source

