Results 321 to 330 of about 752,311 (360)
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Drug Discovery Today, 2004
Abstract Many ion channels are recognized as amenable targets for a range of disease states and conditions. However, the process of discovering drugs is highly influenced by the chemical doability, the biological confidence in rationale of the approach and the ‘screenability’.
Christine Williams +2 more
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Abstract Many ion channels are recognized as amenable targets for a range of disease states and conditions. However, the process of discovering drugs is highly influenced by the chemical doability, the biological confidence in rationale of the approach and the ‘screenability’.
Christine Williams +2 more
openaire +3 more sources
2006
Publisher Summary The most common application of the patch-clamp technique is to manipulate the cell membrane voltage and measure the electrical currents that flow across the membrane through ionic channels. Currents can be recorded either from individual channels or from an entire cellular population of channels. If a cell has long processes, such as
Fiona Halliday +2 more
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Publisher Summary The most common application of the patch-clamp technique is to manipulate the cell membrane voltage and measure the electrical currents that flow across the membrane through ionic channels. Currents can be recorded either from individual channels or from an entire cellular population of channels. If a cell has long processes, such as
Fiona Halliday +2 more
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[9] Patch voltage clamping with low-resistance seals: Loose patch clamp
1992Publisher Summary This chapter discusses several loose-seal methods, and emphasizes the possible difficulties associated with each, describing how they can be avoided. The chapter also discusses applications in which the potential across the membrane is controlled.
Wolfhard Almers, William M. Roberts
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Force-controlled patch clamp of beating cardiac cells.
Nano letters (Print), 2015From its invention in the 1970s, the patch clamp technique is the gold standard in electrophysiology research and drug screening because it is the only tool enabling accurate investigation of voltage-gated ion channels, which are responsible for action ...
D. Ossola +5 more
semanticscholar +1 more source
1995
In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology.
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In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology.
openaire +3 more sources
2020
Intact and functioning brain enables quantification of neural activities directly associated with real world such as visual and auditory information. In vivo patch clamp can record different types of neuronal activity, such as spiking responses, membrane potential dynamics, and synaptic currents (e.g., EPSC, IPSC) in either anesthetized or awake or ...
Zhongju Xiao, Yi Zhou, He Li
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Intact and functioning brain enables quantification of neural activities directly associated with real world such as visual and auditory information. In vivo patch clamp can record different types of neuronal activity, such as spiking responses, membrane potential dynamics, and synaptic currents (e.g., EPSC, IPSC) in either anesthetized or awake or ...
Zhongju Xiao, Yi Zhou, He Li
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1983
Ion-channel reconstitution and patch recording offer different but complementary experimental methods. Both can be used to help provide a molecular understanding of how ion transport activity is controlled by protein structure and regulation. On the one hand, through use of reconstitution techniques, an ion channel can be isolated, purified, and/or ...
Christopher Miller, David W. Tank
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Ion-channel reconstitution and patch recording offer different but complementary experimental methods. Both can be used to help provide a molecular understanding of how ion transport activity is controlled by protein structure and regulation. On the one hand, through use of reconstitution techniques, an ion channel can be isolated, purified, and/or ...
Christopher Miller, David W. Tank
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2013
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as ...
Clemens Möller, Carol J. Milligan
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Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as ...
Clemens Möller, Carol J. Milligan
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A patch-clamp study of Bacillus subtilis
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1992In patch-clamp experiments on giant protoplasts of the Gram-positive bacterium Bacillus subtilis, membrane stretch resulted in an initial transient collapse of the membrane resistance, after which stretch-activated, voltage modulated, high-conductance channels could be observed.
SZABO', ILDIKO' +2 more
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2013
Patch clamping was first introduced into biophysical studies by Neher and Sakmann [1] in 1976 and was soon expanded to many other fields such as biology and medicine. The technique not only allowed the detection of single-channel currents in biological membranes for the first time but also enabled higher current resolution, direct membrane patch ...
Kyle Jiang, Majid Malboubi
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Patch clamping was first introduced into biophysical studies by Neher and Sakmann [1] in 1976 and was soon expanded to many other fields such as biology and medicine. The technique not only allowed the detection of single-channel currents in biological membranes for the first time but also enabled higher current resolution, direct membrane patch ...
Kyle Jiang, Majid Malboubi
openaire +2 more sources