Results 81 to 90 of about 35,849 (293)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Cell Division Mode Change Mediates the Regulation of Cerebellar Granule Neurogenesis Controlled by the Sonic Hedgehog Signaling

Stem Cell Reports, 2015
Symmetric and asymmetric divisions are important for self-renewal and differentiation of stem cells during neurogenesis. Although cerebellar granule neurogenesis is controlled by sonic hedgehog (SHH) signaling, whether and how this process is mediated by
Rong Yang, Minglei Wang, Jia Wang, Xingxu Huang, Ru Yang, Wei-Qiang Gao   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Novel single-step microfluidic strategies for production of patched and Janus bipolymer microparticles [Abstract] [PDF]

, 2016
Novel single-step microfluidic strategies for production of patched and Janus bipolymer microparticles ...
Ekanem Ekanem (1259004), Zilin Zhang (4370773), Goran Vladisavljevic (1253448)   +2 more
core   +3 more sources

Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception

eLife, 2014
Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development.
Shen Yue, Liu-Ya Tang, Ying Tang, Yi Tang, Qiu-Hong Shen, Jie Ding, Yan Chen, Zengdi Zhang, Ting-Ting Yu, Ying E Zhang, Steven Y Cheng   +10 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A crew of patches [PDF]

Current Biology, 1998
Given the present obsession with pattern formation, it is hard to appreciate that 30 years ago there were but few eccentrics who knew what the words meant. In those days, any who tried to understand patterns searched throughout zoology and botany for colleagues who shared their interest.
openaire   +2 more sources

Automated Mapping of Patched Cropland Parcels Using Bi-Temporal UAS Elevation and Spectral Features at Cadastral Level

Remote Sensing
Cropland parcels are fundamental units in agricultural production, and their precise delineation is critical for cadastral management and precision agriculture.
Xiaoshan Yong, Jianyong Zhang, Yu Zhao, Qian Cui, Shijie Qiao, Yanjie Liu, Yugang Cao, Wu Xiao   +7 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Design of Flyby Trajectories with Powered Gravity and Aerogravity Assist Maneuvers

Aerospace
In this paper, we investigate flyby trajectories combining powered gravity assist (PGA) with aerogravity assist (AGA) in the planar elliptic restricted three-body problem (PERTBP). The patched flyby trajectory is divided into three portions: the PGA, AGA
Wanze Yu, Yi Qi
doaj   +1 more source