Results 81 to 90 of about 464,280 (313)

Evaluation of Real-Time PCR Coupled With Multiplex Probe Melting Curve Analysis for Pathogen Detection in Patients With Suspected Bloodstream Infections

Frontiers in Cellular and Infection Microbiology, 2019
Background: This study aimed to evaluate real-time polymerase chain reaction coupled with multiplex probe melting curve analysis (PCR-MCA) for pathogen detection in patients with suspected bloodstream infections (BSIs).Methods: A PCR-MCA assay was ...
Yao Xiao, Yao Xiao, Xu Shen, Qi-Feng Zhao, Qi-Feng Zhao, Yi-Hui Yao, Tian-Ci Yang, Tian-Ci Yang, Jian-Jun Niu, Jian-Jun Niu   +9 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire [PDF]

, 2010
Background: Pythium ultimum (P. ultimum) is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. Results: The P.
Beakes, Gordon W, Lévesque, CA, Cano, L., Puiu, D, Ronald P De Vries, Raffaele, Sylvain, Hostetler, Jessica, Henrissat, B., Paul D Thomas, Phuntmart, Vipaporn, Boore, J.L., Brett R Whitty, Beakes, GW, Whitty, Brett R, Grenville-Briggs, L., Whitty, Brett R., Horner, N, Cano, L, Raffaele, S., Robideau, Gregg P, Steve Ferriera, Tisserat, Ned, Shetty, J, Tisserat, N., Busam, D, Krajaejun, T., Stajich, JE, Holt, C, Yandell, M, Zerillo, MM, C Robin Buell, West, Pieter van, Morris, P., Hamilton, John P, Pedro M Coutinho, Buell, C. Robin, Martin, Frank, Wawra, S, Whitty, B.R., Sucheta Tripathy, Meijer, Harold J. G., Thines, Marco, Buell, C.R., Wawra, Stephan, Win, J, Gregg P Robideau, Gordon W Beakes, Gachon, Claire M. M., Henk Brouwer, Gachon, C.M.M., Ned Tisserat, Susan I Fuerstenberg, Whitty, BR, Busam, Dana, Phuntmart, V., Thomas, P.D., Govers, F, Mark Yandell, Hostetler, J, Marcelo M Zerillo, Gachon, Claire MM, Lévesque, C.A., Neil Horner, Beakes, G.W., Tisserat, N, Dana Busam, Dumas, B., Yandell, M., Horner, Neil, Krajaejun, T, Zerillo, M.M., Joe Win, Edgar Huitema, Tyler, Brett M., Robideau, G.P., Zerillo, M., Zerillo, Marcelo M, Grenville-Briggs, L, Krajaejun, Theerapong, Raffaele, S, Vries, Ronald Peter de, Johnson, J, Theerapong Krajaejun, Coutinho, Pedro, M, Lévesque, C André, Yandell, Mark, Govers, F., Vries, R.P., de, van West, Pieter, Rays HY Jiang, Puiu, Daniela, Boore, JL, Beakes, Gordon W., De Vries, Ronald P., Coutinho, P.M., Gaulin, Elodie, Win, J., Barry Moore, van West, P, Meijer, H.J.G., Meijer, HJG, Lin, H., Wawra, S., Lin, H, Jessica Hostetler, Paul Morris, Lévesque, C. André, Stephan Wawra, Sylvain Raffaele, Laura Grenville-Briggs, Fuerstenberg, S.I., Bernard Dumas, Meijer, Harold JG, Shetty, J., Phuntmart, V, De Vries, R.P., Tripathy, S, Coutinho, Pedro M., Gachon, Claire M M, Moore, B, Thines, M, Claire MM Gachon, Fuerstenberg, SI, Robideau, Gregg P., Henrissat, Bernard, Dumas, B, Huitema, Edgar; id_orcid, Brouwer, Henk, Grenville-Briggs, Laura, Moore, Barry, Hostetler, J., Huitema, E., Liliana Cano, Puiu, D., Coutinho, Pedro,, Hamilton, J.P., Ferriera, Steve, Zerillo, Marcelo M., Marco Thines, Daniela Puiu, Huitema, E, Sophien Kamoun, Henrissat, B, Boore, Jeffrey L., Jiang, Rays HY, Stajich, Jason E, De Vries, Ronald P, Bernard Henrissat, van West, P., Kamoun, Sophien, Gaulin, E, Brouwer, H., Win, Joe, Gaulin, E., West, P., van, Huitema, Edgar, Brouwer, H, Justin Johnson, Jiang, Rays H. Y., Thines, M., Jyoti Shetty, Tripathy, S., Frank Martin, Tyler, Brett M, Buell, C Robin, Jiang, RHY, Buell, CR, Morris, Paul, Govers, Francine, Tyler, B.M., Jason E Stajich, Ferriera, S, Jiang, R.H.Y., John P Hamilton, Martin, F, Meijer, Harold J G, Kamoun, S., Jiang, Rays H Y, Brett M Tyler, Fürstenberg, Susan, Hamilton, John P., Lin, Haining, Horner, N., Elodie Gaulin, Thomas, Paul D, Haining Lin, Robideau, GP, Coutinho, PM, Johnson, Justin, Tyler, BM, Harold JG Meijer, Francine Govers, Fuerstenberg, Susan I, Cano, Liliana, Vipaporn Phuntmart, Stajich, J.E., Moore, B., Holt, Carson, Thomas, Paul D., Holt, C., Hamilton, JP, Gachon, CMM, Shetty, Jyoti, Stajich, Jason E., Carson Holt, Jeffrey L Boore, Coutinho, Pedro M, Martin, F., Boore, Jeffrey L, De Vries, RP, Johnson, J., Pieter van West, Morris, P, Thomas, PD, C André Lévesque, Fuerstenberg, Susan I., Dumas, Bernard, Ferriera, S., Tripathy, Sucheta, Kamoun, S, Busam, D.   +220 more
core   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Advancements in Pathogen Detection: Argonaute-Based Nucleic Acid Detection Technology

Pathogens
In recent years, global public health security has encountered significant challenges, with infectious diseases accounting for approximately 25% of global mortality annually.
Meng Hong, Guodi Wu, Yanli Ren, Shanshan Wu, Haihong Zhu, Zhi Chen   +5 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Advances and Innovations in Conjugated Polymer Fluorescent Sensors for Environmental and Biological Detection

Biosensors
Thanks to their multiple outstanding features—such as high fluorescence quantum yield, good photostability, and excellent sensitivity—conjugated polymers (CPs) have emerged as a pioneering class of fluorescent materials for sensing applications ...
Viet-Duc Phung, Vinh Van Tran
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Cronobacter, the emergent bacterial pathogen Enterobacter sakazakii comes of age; MLST and whole genome sequence analysis [PDF]

, 2014
Background: Following the association of Cronobacter spp. to several publicized fatal outbreaks in neonatal intensive care units of meningitis and necrotising enterocolitis, the World Health Organization (WHO) in 2004 requested the establishment of a ...
Keith A Jolley, Forsythe, SJ, Stephen J Forsythe, Jolley, KA, Benjamin Dickins, Dickins, B   +5 more
core   +1 more source

Laboratory Validation of a Fully Automated Point-of-Care Device for High-Order Multiplexing Real-Time PCR Detection of Respiratory Pathogens

Diagnostics
Background/Objectives: We have previously reported the engineering of a point-of-care (POC) system that fully automates the procedures for nucleic acid extraction and multiplexed real-time RT-PCR, with a major advantage of high-level multiplexing.
Libby C. W. Li, Deborah M. S. Tai, Anita Yee, Nancy B. Y. Tsui, Parker Y. L. Tsang, Sunny L. H. Chu, Chui Ting Leung, Bernice K. W. Leung, Winston Wong, Firaol Tamiru Kebede, Pete Y. M. Leung, Teresa Chung, Cyril C. Y. Yip, Jonathan H. K. Chen, Rosana W. S. Poon, Kelvin K. W. To, Kwok-Yung Yuen, Manson Fok, Johnson Y. N. Lau, Lok Ting Lau   +19 more
doaj   +1 more source