Results 101 to 110 of about 2,455,447 (352)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Connectivity aware routing - a method for finding bandwidth constrained paths over a variety of network topologies [PDF]

, 2003
Multimedia traffic and real-time e-commerce applications can experience quality degradation in traditional networks such as the Internet. These difficulties can be overcome in networks which feature dynamically set up paths with bandwidth and delay ...
M. Collier, Collier, Martin, Kowalik, Karol, K. Kowalik   +3 more
core   +2 more sources

Fibonacci number of the tadpole graph

Electronic Journal of Graph Theory and Applications, 2014
In 1982, Prodinger and Tichy defined the Fibonacci number of a graph G to be the number of independent sets of the graph G. They did so since the Fibonacci number of the path graph Pn is the Fibonacci number F(n+2) and the Fibonacci number of the cycle ...
Joe DeMaio, John Jacobson
doaj   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Multiple paths through a network [PDF]

The most sophisticated iterative algorithm for balancing network congestion for a given set of desired vehicle movement from origins to destinations can generate thousands of paths of equal cost to connect a single O-D pair.
Harris, Britton
core  

Generating feasible transition paths for testing from an extended finite state machine (EFSM) with the counter problem [PDF]

, 2010
The extended finite state machine (EFSM) is a powerful approach for modeling state-based systems. However, testing from EFSMs is complicated by the existence of infeasible paths.
Stephen Swift, Swift, S, Abdul Salam Kalaji, Kalaji, AS, Hierons, RM, Robert Hierons   +5 more
core   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Homotopy Path Spaces for Families of Admissible Paths

Journal of Dynamical and Control Systems, 2016
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Jimmie Lawson, Eyüp Kizil
openaire   +3 more sources