Results 141 to 150 of about 154,598 (309)

Expression of ADRB2 in human PBMCs, enrichement of pDCs.

, 2013
(A, B) PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against CD123, CD304, CD14 and ADRB2 or an isotype control, cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ...
Andreas Hoeft (33118), Tobias Hilbert (416028), Jens M. Poth (416032), Christina Weisheit (416030), Georg Baumgarten (33122), Josef Bongartz (416029), Pascal Knüfermann (416031)   +6 more
core   +1 more source

RNA‐Binding Protein RBM25 Targets the mRNA Stability of GTPase Rab22a to Restrict Viral Entry and Infection

Advanced Science, EarlyView.
This study identifies RNA‐binding protein RBM25 as a broad‐spectrum antiviral factor acting independently of type I interferon. It blocks viral entry by suppressing GTPase Rab22a via the RC3H1‐mediated destabilization of Rab22a mRNA. Viral downregulation of RBM25 enhances GTPase Rab22a expression and viral entry, revealing an unreported post ...
Yingying Ding, Huiying Chen, Yuyu Jiang, Chunyan Zhao, Jie Bai, Yan Xiang, Zeting Wang, Xixi Wang, Bing Rui, Wanda Tang, Yue Ding, Zhenzhen Zhan, Yunkai Zhang, Xingguang Liu   +13 more
wiley   +1 more source

Occult HBV infection in PBMCs of HIV+ individuals

, 2010
Backgrund and aim: Detection of HBV-DNA in HBsAg negative patients is defined as occult HBV infection. Previously, we investigated the prevalence and the genomic heterogeneity of occult HBV in the liver of 24 HIV+/HbsAg negative patients: 8 HBsAg ...
DE MITRI, MARIA STELLA, I. Santeramo, CASSINI, ROMINA, VERUCCHI, GABRIELLA, GIBELLINI, DAVIDE, G. Lettini   +5 more
core  

Levels of SMN protein in PBMCs and fibroblasts.

, 2016
Levels of SMN protein in PBMCs and fibroblasts.
Yana van der Weegen (3366536), Dennis Dooijes (119429), Camiel A. Wijngaarde (3366554), Kees P. J. Braun (397362), Renske I. Wadman (3366548), Marc D. Jansen (596869), Peter Sodaar (3366545), Marloes Stam (3366551), Oliver Harschnitz (3366539), W. Ludo van der Pol (3366557), Leonard H. van den Berg (119440), Henny H. Lemmink (3366542)   +11 more
core   +1 more source

Condition‐Associated Pattern Extraction and Recovery From Multi‐Condition Single‐Cell RNA‐seq Data With CAPER

Advanced Science, EarlyView.
Decoupling biological signals from unwanted variation in multi‑condition single‑cell RNA sequencing data remains challenging. CAPER disentangles condition‑associated biological effects from sample heterogeneity through matrix factorization, producing interpretable latent factors and a batch‑corrected expression matrix.
Ye Li, Jin Ning, An Wang, Minxi Shi, Yuanze Chen, Guoliang Liu, Shiquan Sun   +6 more
wiley   +1 more source

Comparing PBMC isolation approaches and assessing epigenetic immune cell quantification as quality control for PBMCs

PLOS One
Ex vivo stability of whole blood is limited. To conserve the mononuclear cell fraction, PBMCs are isolated, stored and serve as substrate for downstream analyses. The quality of PBMCs depends on the purification procedure. Thereby, anti-coagulants, collection tubes, storage temperature and time before isolation, as well as the separation matrix and ...
Lukas Heller, Isabell Janack, Konstantin Schildknecht, Udo Baron, Sven Olek   +4 more
openaire   +3 more sources

CSFV interference in the PBMCs from superinfected hosts.

, 2016
CSFV interference in the PBMCs from superinfected hosts.
Sara Muñoz-González (734156), Marta Pérez-Simó (2503945), Andreu Colom-Cadena (2503939), Lester Josué Pérez (734157), Mariano Domingo (320861), Rosa Rosell (294966), Llilianne Ganges (424889), Oscar Cabezón (294965), Ignasi Marco (294968), Santiago Lavín (293723), José Alejandro Bohórquez (2503942)   +10 more
core   +1 more source

An Integrative Strategy Delineates Modular Metabolic Remodeling and Potential Therapeutic Targets Across Metabolic Diseases

Advanced Science, EarlyView.
An integrative single‐cell atlas across multiple metabolic diseases reveals coordinated metabolic modules and disease‐shared versus disease‐specific pathway activities. By systematically comparing scoring strategies, a robust RankAve framework is established. Coupled with network analysis and drug‐target prediction, this resource uncovers cross‐disease
Kuan Yang, Congxue Hu, Hang Zhao, Xiao Peng, Yiming Yan, Jiasi Wang, Wenqi Jiang, Depeng Mu, Yan Cao, Xia Li, Yunpeng Zhang   +10 more
wiley   +1 more source

MiR-423-5p expression in PBMCs (n = 3).

, 2019
(A) MiR-425-5p expression in PBMCs of CAD, AMI_T0 and AMI_T1 patients. Kruskal-Wallis statistic 11.93, p≤0.005. Discriminatory power of miR-423-5p expression in PBMCs.
Ruggiero Mango (266696), Massimiliano Macrini (6667886), Elena Morini (6667871), Francesca Amati (185039), Chiara Vancheri (6667874), Gianluca Massaro (6667880), Giuseppe Novelli (76046), Barbara Rizzacasa (6667868), Sara Maletta (6667883), Francesco Romeo (126298), Silvio D’Annibale (6667889), Simone Budassi (6667877)   +11 more
core   +1 more source

Engineering Human Donor Derived Germinal Center‐Like Organoids (GCLOs) for Studying Immune Response to Vaccination

Advanced Science, EarlyView.
We bioengineered a new approach methodology of generating Germinal Center Organoids that self‐organize from human blood‐derived immune cells. These immune organoids reproduce key features of humoral immunity, IgG production, and plasmablast emergence.
Bhumi Suthar, Carlos Gomez, Giancarlo Asencio, Suresh Pallikkuth, Ashutosh Agarwal   +4 more
wiley   +1 more source