Results 51 to 60 of about 154,598 (309)
Cytokine production by ASA-stimulated PBMCs.
, 2013 PBMCs were isolated from the blood of ASA-sensitized pigs at the indicated times and stimulated with ASA. The frequencies of ASA-specific IFN-γ secreting PBMCs (A) and the quantities of IL-10 secreted by the PBMCs (B) were determined using an ELISPOT ...
Debra J. Thomas (355440) +5 more
core +1 more source
Molecular Oncology, EarlyView.Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena +15 more
wiley +1 more source
PBMC miRNA in diagnosis of rheumatoid arthritis
International journal of health sciences, 2022 Background and objective: RA is an autoimmune disorder mediated by autoantibodies. miRNAs have been shown to negatively regulate protein translation via an antisense RNA-RNA interaction. Altered miRNA expression has been well established in autoimmune diseases. In this study, the expression of four microRNA was analysed.
Nishtha Agarwal +5 more
openaire +1 more source
Comparative biochemical analysis between brain and PBMCs.
, 2013 A) Immunoblots of PrPC glycoforms from PBMCs and brain, Denatured lysates from 106 PBMCs and 10% brain were tested by Western Blot analysis using 2 antibodies: SAF32 (79–91), 3F4 (109–112). The 35–37 kDa band corresponds to the M.W. of the diglycosylated
Sylvain Lehmann (372399) +2 more
core +1 more source
Molecular Oncology, EarlyView.Radiotherapy (RT) response depends on the DNA repair capacity of tumor and host cells. We show that circulating tumor cell (CTC) counts and apoptosis rates before and after RT predict treatment response and outcome, which can be accessed via easily accessible liquid biopsy approaches. Created in BioRender. Wikman, H.
Yvonne Goy +10 more
wiley +1 more source
BMC Immunology, 2022 Human peripheral blood mononuclear cells (PBMCs) originate from hematopoietic stem cells in the bone marrow, which mainly includes lymphocytes (T cells, B cells, and natural killer cells) and monocytes. Cryopreserved PBMCs providing biobank resources are
Bo Li +8 more
doaj +1 more source
, 2017 Commonly used protocol to isolate peripheral blood mononuclear cells from whole human blood or apheresis ...
Girija Goyal, Jaclyn Long
openaire +1 more source
PBMC Thawing Protocol v1 [PDF]
, 2020 This protocol details methods for thawing peripheral blood mononuclear cells (PBMC). For a protocol detailing Culture and Stimulation, please view the following: PBMCs Culture and Stimulation. For a protocol detailing Cell Staining for Flow Cytometry Assay, please view the following: Cell Staining for Flow Cytometry Assay.
openaire +1 more source
HTRF analysis of soluble huntingtin in PHAROS PBMCs [PDF]
Neurology, 2013 We measured the levels of mutant huntingtin (mtHtt) and total huntingtin (tHtt) in blood leukocytes from Prospective Huntington At-Risk Observational Study (PHAROS) subjects at 50% risk of carrying the Huntington disease mutation using a homogeneous time-resolved fluorescence (HTRF) assay to assess its potential as a biomarker.Peripheral blood ...
Miriam, Moscovitch-Lopatin +10 more
openaire +2 more sources
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source