Results 91 to 100 of about 769,066 (216)

Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

, 2006
A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp.
SUZZI, G, VAN SINDEREN, D, L. SETTANNI, CORSETTI, Aldo, D. VAN SINDEREN, PAPARELLA, A, CORSETTI, A., SETTANNI, Luca, S. VALMORRI, SUZZI, Giovanna, PAPARELLA, Antonello, VALMORRI, S   +11 more
core   +1 more source

Combination of culture-independent and culture-dependent molecular methods for the determination of bacterial community of iru, a fermented Parkia biglobosa seeds.

Frontiers in Microbiology, 2013
In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques.
Gbenga Adedeji Adewumi, Gbenga Adedeji Adewumi, Gbenga Adedeji Adewumi, Folarin Anthony Oguntoyinbo, Santosh eKeisam, Wahengbam eRomi, Kumaraswamy eJeyaram   +6 more
doaj   +1 more source

INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA

, 2012
Culture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010.
Wu, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China., Zhou, Qiaohong, Wu, Zhenbin, Hu, Shenghua, Cai, Linlin, He, Feng, Kong, Lingwei   +6 more
core  

16S rRNA gene primers used for PCR-DGGE fingerprinting.

, 2013
16S rRNA gene primers used for PCR-DGGE fingerprinting.
Reinhard Ruckser (337512), Angelika Pointner (337507), Marlene Remely (337510), Cornelia Lassl (337503), Alexander G. Haslberger (337513), Berit Hippe (337505), Jutta Zwielehner (337502), Olivier J. Switzeny (337509), Elvira Kitzweger (337511)   +8 more
core   +1 more source

Rapid Detection of rpoB Mutations in Rifampin Resistant M. tuberculosis from Sputum Samples by Denaturing Gradient Gel Electrophoresis

International Journal of Medical Sciences, 2012
Objective: To establish a rapid detection method for identifying rpoB mutations associated with rifampin (RIF) resistance in sputum specimens.Methods: We detected rpoB mutations directly in 90 sputum specimens collected from suspected tuberculosis ...
Jun Li, Jiaojiao Xin, Liyuan Zhang, Longyan Jiang, Hongcui Cao, Lanjuan Li
doaj  

Diversity of Yeasts and Molds by Culture-Dependent and Culture-Independent Methods for Mycobiome Surveillance of Traditionally Prepared Dried Starters for the Production of Indian Alcoholic Beverages

Frontiers in Microbiology, 2018
Marcha, thiat, dawdim, hamei, humao, khekhrii, chowan, and phut are traditionally prepared dried starters used for production of various ethnic alcoholic beverages in North East states of India.
Shankar Prasad Sha, Mangesh Vasant Suryavanshi, Mangesh Vasant Suryavanshi, Kunal Jani, Avinash Sharma, Yogesh Shouche, Jyoti Prakash Tamang   +6 more
doaj   +1 more source

MOLECULAR IDENTIFICATION OF YEAST OF THE PULQUE BY PCR-DGGE, A TRADITIONAL MEXICANBEVERAGE

International Journal of Research -GRANTHAALAYAH, 2015
Currently it is well known that yeasts play an essential role in the production of different beverages. In this paper, were identified some of the yeasts involved in the fermentation process of the pulque, a Mexican traditional beverage. Samples were collected from different regions of Mexico and yeasts were detected directly from samples without ...
Marcial-Quino J., Garcia-Ocón B., Mendoza-Espinoza J.A., Gómez-Manzo S., Sierra-Palacios E   +4 more
openaire   +2 more sources

Group specific RT-PCR DGGE analysis.

, 2014
RT-PCR DGGE analysis of metabolically active free-living (0.22–8 μm in 2006 and 0.22–5 μm in 2008 and 2009) and particle-attached (>8 or >5 μm, respectively) Betaproteobacteria, Actinobacteria, and Sphingomonadaceae after 1O2 and H2O2 exposure.
Stefanie P. Glaeser (542828), Bork A. Berghoff (424746), Verena Stratmann (542829), Hans-Peter Grossart (203577), Jens Glaeser (479943)   +4 more
core   +1 more source

Development and application of tuf gene-based PCR and PCR-DGGE methods for the detection of 16 Bifidobacterium species [PDF]

, 2013
A total of 16 Bifidobacterium species were assayed by polymerase chain reaction (PCR) and PCR–denaturing gradient gel electrophoresis (PCR–DGGE) methods targeted on a 770-bp region of the tuf gene.
Lin, Wen-Hsin, Chen, Hsin-Chih, Chiang, Yu-Cheng, Sheu, Sen-Je, Lin, Chien-Ku, Hwang, Wen-Zhe, Tsen, Hau-Yang   +6 more
core   +1 more source

Characterization of spoilage bacteria in pork sausage by PCR-DGGE analysis

Food Science and Technology, 2013
To investigate microbial diversity and identify spoilage bacteria in fresh pork sausages during storage, twelve industrial pork sausages of different trademarks were stored at 4 °C for 0, 14, 28 and 42 days, 80% relative humidity and packaged in sterile plastic bags. Microbiological analysis was performed. The pH and water activity (a w ) were measured.
Dias, Francesca Silva, Ramos, Cíntia Lacerda, Schwan, Rosane Freitas   +2 more
openaire   +4 more sources