Results 71 to 80 of about 769,066 (216)

Diversity of endophytic bacteria of Dendrobium officinale based on culture-dependent and culture-independent methods

Biotechnology & Biotechnological Equipment, 2017
Culture-dependent and culture-independent methods were compared and evaluated in the study of the endophytic diversity of Dendrobium officinale. Culture-independent methods consisted of polymerase chain reaction–denaturing gradient gel electrophoresis ...
Cong Pei, Chunyi Mi, Lihua Sun, Wenhong Liu, Ou Li, Xiufang Hu   +5 more
doaj   +1 more source

Clinical Validation of Digital PCR for Precise m.3243A>G Heteroplasmy Quantification in Early‐Onset Diabetes

Journal of Diabetes Research, Volume 2026, Issue 1, 2026.
Background Mitochondrial DNA m.3243A>G variant causes maternally inherited diabetes with deafness. We developed a droplet digital PCR (dPCR) assay to quantify m.3243A>G heteroplasmy and investigated its prevalence in Chinese Han early‐onset diabetes patients.
Xiaomu Kong, Lei Bei, Peng Gao, Lixia Lu, Haoyan Zhu, Yongwei Jiang, Meimei Zhao, Yi Liu, Guozhen Wang, Mo Li, Ziyi Feng, Guoxiong Deng, Yongtong Cao, Liang Ma, Hannah Wesley   +14 more
wiley   +1 more source

Exogenously applied ferulic acid and p-coumaric acid differentially affect cucumber rhizosphere Trichoderma spp. community structure and abundance

Plant, Soil and Environment, 2020
Continuous monocropping can cause the buildup of autotoxins (e.g., phenolic compounds) in the soil, which can alter soil microbial community and inhibit plant growth.
Muhammad Khashi U Rahman, Shengcheng Tan, Changli Ma, Fengzhi Wu, Xingang Zhou   +4 more
doaj   +1 more source

Microbiome‐Based Characterization of Microbial Community Structure and Diversity in White Tea (Camellia sinensis)

Journal of Food Biochemistry, Volume 2026, Issue 1, 2026.
Introduction Aging storage plays a crucial role in enhancing both the flavor and health benefits of white tea. However, the microbial mechanisms underlying this process remain poorly understood, particularly regarding intervarietal differences in microbial succession.
Sihui Xie, Zhengjie Chang, Can Zhang, Anupama Bose   +3 more
wiley   +1 more source

Evaluation of different primers for PCR-DGGE analysis of cheese-associated enterococci

Journal of Dairy Research, 2009
Enterococci represent an important part of bacterial microbiota in different types of artisanal cheeses, made from either raw or pasteurized milk. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) of ribosomal DNA is currently one of the most frequently used fingerprinting method to study diversity and dynamics of microbial ...
Mohar Lorbeg, Petra, Čanžek Majhenič, Andreja, Rogelj, Irena   +2 more
openaire   +3 more sources

Genetic Confirmations of Dwarf Lemurs Across Madagascar Highlights Complex Biogeographic Patterns 马达加斯加全境鼠狐猴的遗传确认揭示了复杂的生物地理模式

Integrative Conservation, Volume 4, Issue 4, Page 665-674, December 2025.
Using a diagnostic mitochondrial marker gene, we document the presence of furry‐eared dwarf lemurs, a species known from Madagascar's eastern montane rainforests, in several forest fragments across the island's central highlands. These findings raise new questions about when this species colonized these sites, how they tolerate these changing and ...
Marina B. Blanco, Lydia K. Greene, Antonin Andriamahaihavana, Jean‐Basile Andriambeloson, Radosoa Andrianaivoarivelo, Herizo Andrianantenaina, Ando Andriantsalohimisantatra, Landry Bemita, Chris Birkinshaw, Jean Marcellin Dimbilahy, Elaine E. Guevara, Anna Mananjara Mahefaniaina, Manitra F. Rajaonarison, Toky H. Rakotoarinivo, Fenonirina Rakotoarison, Lovasoa Sylviane Rakotozafy, Elodi Rambeloson, Tianjanahary Randriamboavonjy, Yockline Randriamihanta, Hoby A. Rasoanaivo, Rojo N. Ravelojaona, Josia Razafindramanana, Sandratra R. Rakotonomenjanahary, Tahiry V. Rivoharison, Haja R. Tongasoa, Rachel C. Williams, Fanomezana M. Ratsoavina, Anne D. Yoder   +27 more
wiley   +1 more source

Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA [PDF]

, 2001
The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Anneke Keijzer, Van Elsas, J.D., Duineveld, B.M., Kowalchuk, G.A., Van Veen, J.A., George A. Kowalchuk, Jan Dirk van Elsas, Keijzer, A., Veen, J.A., van, Bernadette M. Duineveld, Elsas, J.D., van, Johannes A. van Veen   +11 more
core   +1 more source

Lurking in the leaves: How large native trees and leaf litter promote amphibian diversity in Malagasy agroforests

Journal of Applied Ecology, Volume 62, Issue 12, Page 3395-3407, December 2025.
While old‐growth forests are irreplaceable for conserving Madagascar's endemic amphibian diversity, agroforests can also support amphibian populations, especially if management enhances habitat complexity through increased leaf litter and the presence of large native trees.
Lovasoa Manuelle Sylviane Rakotozafy, Ursina Tobler, Romual Randriamanantena, Kiady Sedera Rasataharifaly, Aristide Andrianarimisa, Dominic Andreas Martin   +5 more
wiley   +1 more source

Use of Haploid Mixtures and Heteroduplex Analysis Enhance Polymorphisms Revealed by Denaturing Gradient Gel Electrophoresis

BioTechniques, 2000
PCR-based codominant genetic markers were developed by using primer sequences designed from cDNA clones of loblolly pine (Pinus taeda L.). Such markers offer certain advantages relative to simple-sequence repeat (SSR), also known as short-tandem repeat ...
Berhanu Temesgen, David B. Neale, David E. Harry   +2 more
doaj   +1 more source

PCR and DGGE detection limits for wine spoilage microbes [PDF]

, 2010
The original publication is available at http://www.sasev.org/.In this study the culture-independent technique, polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE), was investigated for the early detection and identification of
Witthuhn, R. C., Bester, L., Cameron, M., Du Toit, M. D.   +3 more
core