Results 51 to 60 of about 7,749 (169)

Determination of Transgene Copy Number and Expression Level Using Denaturing Gradient Gel Electrophoresis

open access: yesBioTechniques, 1998
Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models.
M.D. Ringel   +4 more
doaj   +1 more source

Fungal Diversity and Evaluation of Ochratoxin A Content of Coffee from Three Cameroonian Regions

open access: yesJournal of Food Quality, 2020
The present study had the objective to assess the ochratoxin A content of coffee through chromatographic analysis and design a method using PCR-DGGE to analyze at the same moment the totality of fungal flora present in the coffee samples in order to ...
N. D. Nganou   +5 more
doaj   +1 more source

Clinical Validation of Digital PCR for Precise m.3243A>G Heteroplasmy Quantification in Early‐Onset Diabetes

open access: yesJournal of Diabetes Research, Volume 2026, Issue 1, 2026.
Background Mitochondrial DNA m.3243A>G variant causes maternally inherited diabetes with deafness. We developed a droplet digital PCR (dPCR) assay to quantify m.3243A>G heteroplasmy and investigated its prevalence in Chinese Han early‐onset diabetes patients.
Xiaomu Kong   +14 more
wiley   +1 more source

Microbiome‐Based Characterization of Microbial Community Structure and Diversity in White Tea (Camellia sinensis)

open access: yesJournal of Food Biochemistry, Volume 2026, Issue 1, 2026.
Introduction Aging storage plays a crucial role in enhancing both the flavor and health benefits of white tea. However, the microbial mechanisms underlying this process remain poorly understood, particularly regarding intervarietal differences in microbial succession.
Sihui Xie   +3 more
wiley   +1 more source

Genetic Confirmations of Dwarf Lemurs Across Madagascar Highlights Complex Biogeographic Patterns 马达加斯加全境鼠狐猴的遗传确认揭示了复杂的生物地理模式

open access: yesIntegrative Conservation, Volume 4, Issue 4, Page 665-674, December 2025.
Using a diagnostic mitochondrial marker gene, we document the presence of furry‐eared dwarf lemurs, a species known from Madagascar's eastern montane rainforests, in several forest fragments across the island's central highlands. These findings raise new questions about when this species colonized these sites, how they tolerate these changing and ...
Marina B. Blanco   +27 more
wiley   +1 more source

Diversity of endophytic bacteria of Dendrobium officinale based on culture-dependent and culture-independent methods

open access: yesBiotechnology & Biotechnological Equipment, 2017
Culture-dependent and culture-independent methods were compared and evaluated in the study of the endophytic diversity of Dendrobium officinale. Culture-independent methods consisted of polymerase chain reaction–denaturing gradient gel electrophoresis ...
Cong Pei   +5 more
doaj   +1 more source

Lurking in the leaves: How large native trees and leaf litter promote amphibian diversity in Malagasy agroforests

open access: yesJournal of Applied Ecology, Volume 62, Issue 12, Page 3395-3407, December 2025.
While old‐growth forests are irreplaceable for conserving Madagascar's endemic amphibian diversity, agroforests can also support amphibian populations, especially if management enhances habitat complexity through increased leaf litter and the presence of large native trees.
Lovasoa Manuelle Sylviane Rakotozafy   +5 more
wiley   +1 more source

Exogenously applied ferulic acid and p-coumaric acid differentially affect cucumber rhizosphere Trichoderma spp. community structure and abundance

open access: yesPlant, Soil and Environment, 2020
Continuous monocropping can cause the buildup of autotoxins (e.g., phenolic compounds) in the soil, which can alter soil microbial community and inhibit plant growth.
Muhammad Khashi U Rahman   +4 more
doaj   +1 more source

Percent‐Level Copper Mineralisation Promoted by Copper‐Tolerant Iron‐Oxidising Bacteria in Circumneutral Mine Drainage

open access: yesEnvironmental Microbiology, Volume 27, Issue 12, December 2025.
A neutrophilic Fe(II)‐oxidizing Gallionellaceae was isolated from circumneutral Cu mine drainage. It mediated Fe(III) biomineralization, resulting in pronounced Cu accumulation (up to 2 wt%) within the solid precipitates. ABSTRACT Geomicrobiology of copper (Cu) and iron (Fe) in circumneutral‐pH drainage from a Japanese Cu mine was investigated, with a ...
Kazuya Tanimoto   +5 more
wiley   +1 more source

Orthophosphate and Residence Time Impacts in Chloraminated Model Distribution Systems

open access: yesAWWA Water Science, Volume 7, Issue 6, November/December 2025.
ABSTRACT The combined effects of orthophosphate addition and residence time on chloramine stability, biofilm development, and nitrification potential in drinking water distribution systems (DWDSs) remain unexplored. Most previous studies examined orthophosphate dose under fixed residence time conditions, leaving a gap in understanding how water age ...
Mahmoud H. Badawy   +4 more
wiley   +1 more source

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