Results 61 to 70 of about 7,749 (169)

Use of Haploid Mixtures and Heteroduplex Analysis Enhance Polymorphisms Revealed by Denaturing Gradient Gel Electrophoresis

BioTechniques, 2000
PCR-based codominant genetic markers were developed by using primer sequences designed from cDNA clones of loblolly pine (Pinus taeda L.). Such markers offer certain advantages relative to simple-sequence repeat (SSR), also known as short-tandem repeat ...
Berhanu Temesgen, David B. Neale, David E. Harry   +2 more
doaj   +1 more source

Evaluation of different primers for PCR-DGGE analysis of cheese-associated enterococci

Journal of Dairy Research, 2009
Enterococci represent an important part of bacterial microbiota in different types of artisanal cheeses, made from either raw or pasteurized milk. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) of ribosomal DNA is currently one of the most frequently used fingerprinting method to study diversity and dynamics of microbial ...
Mohar Lorbeg, Petra, Čanžek Majhenič, Andreja, Rogelj, Irena   +2 more
openaire   +3 more sources

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Journal of Veterinary Research, 2016
Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp.
Szacawa Ewelina, Szymańska-Czerwińska Monika, Niemczuk Krzysztof, Dudek Katarzyna, Woźniakowski Grzegorz, Bednarek Dariusz   +5 more
doaj   +1 more source

Efecto de la inoculación de la cepa Sphingomonas paucimobilis 20006FA sobre la composición de un consorcio bacteriano degradador de fenantreno Effect of the inoculant strain Sphingomonas paucimobilis 20006FA on the bacterial composition of a phenanthrene-degrading consortium

Revista Argentina de Microbiología, 2009
Se estudió el efecto de la inoculación con la cepa Sphingomonas paucimobilis 20006FA sobre la composición bacteriana de un consorcio degradador de fenantreno en cultivos discontinuos (batch) con 8 repiques sucesivos.
L. Madueño, B. M. Coppotelli, I. S. Morelli   +2 more
doaj  

Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles Comunidades bacterianas associadas à rizosfera de cultivares de batata avaliadas por perfis de PCR-DGGE

Pesquisa Agropecuária Brasileira, 2008
The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of ...
Enderson Petrônio de Brito Ferreira, André Nepomuceno Dusi, Gustavo Ribeiro Xavier, Norma Gouvêa Rumjanek   +3 more
doaj   +1 more source

PCR-DGGE Analysis Proves the Suppression of Rhizoctonia and Sclerotium Root Rot Due to Successive Inoculations. [PDF]

J Fungi (Basel), 2022
Elsharkawy MM, Kuno S, Hyakumachi M, Mostafa YS, Alamri SA, Alamri SA, Alrumman SA.   +6 more
europepmc   +1 more source

PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process

Water Science and Technology, 2002
The wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of acids such as nitric acid and of salts. Biological nitrogen removal from this wastewater was attempted by using a circulating bioreactor system equipped with an anoxic packed bed or an anoxic fluidized bed and an aerobic ...
N, Noda, S, Yoshie, T, Miyano, S, Tsuneda, A, Hirata, Y, Inamori   +5 more
openaire   +2 more sources

Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples

Frontiers in Microbiology, 2019
Urinary or ureteral catheter insertion remains one of the most common urological procedures, yet is considered a predisposing factor for urinary tract infection.
Iva Kotaskova, Iva Kotaskova, Iva Kotaskova, Hana Obrucova, Barbora Malisova, Petra Videnska, Barbora Zwinsova, Tereza Peroutkova, Milada Dvorackova, Petr Kumstat, Pavel Trojan, Filip Ruzicka, Veronika Hola, Tomas Freiberger, Tomas Freiberger, Tomas Freiberger   +15 more
doaj   +1 more source

Rapid Detection of rpoB Mutations in Rifampin Resistant M. tuberculosis from Sputum Samples by Denaturing Gradient Gel Electrophoresis

International Journal of Medical Sciences, 2012
Objective: To establish a rapid detection method for identifying rpoB mutations associated with rifampin (RIF) resistance in sputum specimens.Methods: We detected rpoB mutations directly in 90 sputum specimens collected from suspected tuberculosis ...
Jun Li, Jiaojiao Xin, Liyuan Zhang, Longyan Jiang, Hongcui Cao, Lanjuan Li
doaj  

Combination of culture-independent and culture-dependent molecular methods for the determination of bacterial community of iru, a fermented Parkia biglobosa seeds.

Frontiers in Microbiology, 2013
In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques.
Gbenga Adedeji Adewumi, Gbenga Adedeji Adewumi, Gbenga Adedeji Adewumi, Folarin Anthony Oguntoyinbo, Santosh eKeisam, Wahengbam eRomi, Kumaraswamy eJeyaram   +6 more
doaj   +1 more source