Results 201 to 210 of about 3,745,037 (345)

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, Volume 15, Issue 7, Page 1079-1089, July 2025.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Diagnostic utility of real-time RT-PCR for chikungunya virus detection in the acute phase of infection: a retrospective study. [PDF]

Ann Med
Sajith A, Iyengar V, Varamballi P, Mukhopadhyay C, Nittur S.   +4 more
europepmc   +1 more source

Analysis of chromatin structure by ligation-mediated PCR. [PDF]

, 1992
Gerd P. Pfeifer
openalex   +1 more source

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, Volume 15, Issue 7, Page 1090-1102, July 2025.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

Incidence of Homozygous <i>SMN2</i> Deletion in Japan: Cross-Reactivity of <i>SMN2</i> Primers with <i>SMN1</i> Sequence Causes False Negatives in Real-Time PCR Screening. [PDF]

Genes (Basel)
Sakima M, Bouike Y, Wada SI, Nakamae M, Noguchi Y, Bo R, Awano H, Oba J, Nishio H.   +8 more
europepmc   +1 more source

Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element. [PDF]

, 1993
Lillian W. Chiang, Iulia A. Kovari, Martha M. Howe   +2 more
openalex   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, Volume 15, Issue 7, Page 1103-1112, July 2025.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source

Multiplex detection of seven transgenes for human gene doping analysis. [PDF]

Sci Rep
Naumann N, Do C, Vollmert C, Krajina M, Thomas A, Cheung HW, Wong KS, Wan TSM, Ho ENM, Thevis M.   +9 more
europepmc   +1 more source

The production of PCR products with 5′ single-stranded tails using primers that incorporate novel phosphoramidite intermediates [PDF]

, 1993
Charles R. Newton, David A. Holland, Lesley Heptinstall, Ian Hodgson, Michael D. Edge, Alexander F. Markham, Michael J. McLean   +6 more
openalex   +1 more source

KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for Ca2+‐mediated response to dimethylsulfoxide (DMSO)

FEBS Open Bio, Volume 15, Issue 7, Page 1113-1123, July 2025.
Ca2+‐mediated response to DMSO was investigated in Saccharomyces cerevisiae cells expressing Ca2+‐dependent aequorin. Cell exposure to DMSO induced a cytosolic Ca2+ wave dependent on the integrity of the Cch1/Mid1 channel. Deletion of KCS1 or VIP1 genes encoding the phosphoinositol pyrophosphate (PP‐IP) synthases suppressed the DMSO‐induced Ca2 ...
Larisa Ioana Gogianu, Lavinia Liliana Ruta, Claudia Valentina Popa, Simona Ghenea, Ileana Cornelia Farcasanu   +4 more
wiley   +1 more source