Results 201 to 210 of about 3,342,563 (345)

Development of a Real-Time PCR Assay for Identification of Coccidioides immitis by Use of the BD Max System [PDF]

, 2015
Marilyn A. Mitchell, Dominic Dizon, Robert Libke, Michael W. Peterson, David Slater, Akashdeep Dhillon   +5 more
openalex   +1 more source

'Touchdown' PCR to circumvent spurious priming during gene amplification.

Nucleic Acids Research, 1991
R. H. Don, P. Cox, Brandon J Wainwright, K. Baker, J. Mattick   +4 more
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Pathological response to neoadjuvant chemotherapy and survival in patients with breast cancer: A multicentric retrospective study. [PDF]

Oncol Lett
Séka EN, Traoré DA, Saki CT, Traoré K, Tuo AG, Doukouré B.   +5 more
europepmc   +1 more source

Molecular detection of Leishmania species in Sand Flies by PCR-RFLP technique in refugee camps

A. AL‐LAHEM, Reem A. Alajmi, Mais Alzarzor Alajami, Saeed El‐Ashram, Muhammad Amjad Bashir, Rewaida Abdel‐Gaber   +5 more
openalex   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

A pooled RT-PCR testing strategy for more efficient COVID-19 pandemic management

, 2021
Akif Ayaz, Aslı Güner Öztürk Demir, Gürkan Öztürk, Mehmet Koçak   +3 more
openalex   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

The Use of CSF Multiplex PCR Panel in Patients with Viral Uveitis. [PDF]

Diagnostics (Basel)
Jeong YH, Park SH, Lee SM, Byon I, Yi J, Park SW.   +5 more
europepmc   +1 more source

Peer Review #3 of "Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber–pumpkin grafted plants (v0.1)"

, 2019
Johannes Fahrentrapp
openalex   +1 more source