Results 201 to 210 of about 3,602,392 (295)

Implementation of Point-of-Care PCR-testing for the diagnosis of respiratory infections in vulnerable patient populations. [PDF]

PLoS One
Tolle H, Wachinger J, Castro MDM, Morales I, Denkinger CM.   +4 more
europepmc   +1 more source

Novel PCR Methods for Detection of HIV Proviruses Using Degoxigenin Labelled Probes

, 1991
Koushi Yamaguchi, Hiroshi Ushijima, H Tsuchie, Takao Matsumoto, Junichi Matsuda, Takashi Kitamura   +5 more
openalex   +2 more sources

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

FEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda, Yuki Naruse, Yusaku Suizu, Tomohiro Araki, Yoshikazu Hoshi, Haruhiko Sakuraba, Junji Hayashi, Toshihisa Ohshima   +7 more
wiley   +1 more source

Impact of syndromic molecular diagnostics on antimicrobial adequacy and time to therapy in critically ill patients with pneumonia: a systematic review and meta-analysis of randomized trials. [PDF]

Crit Care
de Albuquerque Pessoa Dos Santos Y, Tomazini BM, Dos Santos MHC, de Queiroz EL, Pastore Júnior L, Costa ELV, da Silva Ramos FJ.   +6 more
europepmc   +1 more source

Maximizing sensitivity and specificity of PCR by preamplification heating

, 1991
Richard T. D’Aquila, Lawrence Bechtel, Joseph A. Videler, Joseph J. Eron, Paul Gorczyca, Joan C. Kaplan   +5 more
openalex   +2 more sources

Identification and functional characterization of EFNA3 as a dual diagnostic biomarker and immune therapeutic target in rectal cancer

Advanced Chinese Medicine, EarlyView.
Abstract Background The Eph/ephrin signaling system has emerged as an important regulator of oncogenic processes, yet its specific involvement in rectal adenocarcinoma (READ) pathogenesis requires further elucidation. This study employed an integrative analytical approach combining computational biology with experimental validation to characterize Eph ...
Tianqi Liu, Jiuyuan Fang, Bohan Liu, Hangqi Liu, Yidan Wang, Yipei Jing, Hui Zhang, Huanwen Wu   +7 more
wiley   +1 more source

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR. [PDF]

PLoS One
Phimolsannousith V, Vongsouvath M, Kiedsathid P, Vongsouvath M, Ashley EA, Dubot-Pérès A.   +5 more
europepmc   +1 more source

A Novel CHMP2B Splicing Variant in Atypical Presentation of Familial Frontotemporal Lobar Degeneration

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT C‐truncating variants in the charged multivesicular body protein 2B (CHMP2B) gene are a rare cause of frontotemporal lobar degeneration (FTLD), previously identified only in Denmark, Belgium, and China. We report a novel CHMP2B splice‐site variant (c.35‐1G>A) associated with familial FTLD in Spain. The cases were two monozygotic male twins who
Sara Rubio‐Guerra, Sara Bernal, David Almenta, Josefina Pérez‐Blanco, Valle Camacho, Isabel Sala, Mª Belén Sánchez‐Saudinós, Jesús García Castro, Judit Selma‐González, Miguel Ángel Santos‐Santos, Álvaro Carbayo, Janina Turon‐Sans, Ricard Rojas‐Garcia, Daniel Alcolea, Juan Fortea, Alberto Lleó, Oriol Dols‐Icardo, Ignacio Illán‐Gala   +17 more
wiley   +1 more source

A Novel C19orf47‐AKT2 Chimeric RNA Generated by Cis‐Splicing of Adjacent Genes Is Associated With Glioblastoma Prognosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Malignant gliomas pose significant therapeutic challenges. This study aimed to identify and characterize a novel chimeric RNA in glioma and assess its clinical and functional significance for precision treatment. Methods The C19orf47‐AKT2 chimeric RNAs were identified through RNA sequencing and validated by polymerase chain reaction.
Zihan Wang, Bowen Ni, Kezhi Wu, Qi Zhang, Jinglin Guo, Runwei Yang, Ziyu Wang, Guozhong Yi, Guanglong Huang, Minyi He, Yimin Xu, Yawei Liu   +11 more
wiley   +1 more source