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The impact of the PCR plateau phase on quantitative PCR
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994The quantitative use of the polymerase chain reaction (PCR) is often compromised by the variability of the amplification. The most useful system for quantitation by PCR involves the use of controls which are almost identical to the target and which can be amplified using the same primers as the sequences of interest.
Frank Gannon, Ciaran G. Morrison
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2017
Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor Johny, Sarita G. Bhat
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Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor Johny, Sarita G. Bhat
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2017
Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
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Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
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The polymerase chain reaction (PCR) and RT-PCR
1997The polymerase chain reaction (PCR), a method for the in vitro enzymatic amplification of DNA, has facilitated greatly the analysis of complex genomes by permitting the characterization and cloning of DNA sequences from small samples of DNA or RNA of high sequence complexity. PCR and its specialized variants are now used as widely as any of the ‘older’
ARCA', Bruno, C. Savakis
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Direct PCR of Intact Bacteria (Colony PCR)
Current Protocols in Microbiology, 2016AbstractThis protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
Brian Stevenson +4 more
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PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification
2015The field of molecular biology was revolutionized with the development of the polymerase chain reaction (PCR). This chapter defines PCR, reverse transcription PCR (RT-PCR), real-time PCR, digital PCR and isothermal amplification. Within each subject a brief overview of the process is given along with the required reagents or components and highlighted ...
Janine R. Hutchison +3 more
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2010
Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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cclm, 2000
Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Enzymatic Amplification of RNA by PCR (RT‐PCR)
Current Protocols in Molecular Biology, 2001AbstractMethods for enzymatic amplification of RNA by the polymerase chain reaction (RT‐PCR) are highlighted in this unit. The is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery.
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