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PCR und Real-Time PCR

2010
Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
openaire   +1 more source

CODEHOP PCR and CODEHOP PCR Primer Design

2010
While PCR primer design for the amplification of known sequences is usually quite straightforward, the design, and successful application of primers aimed at the detection of as yet unknown genes is often not. The search for genes that are presumed to be distantly related to a known gene sequence, such as homologous genes in different species, paralogs
Jeannette P, Staheli   +3 more
openaire   +2 more sources

Direct PCR of Intact Bacteria (Colony PCR)

Current Protocols in Microbiology, 2008
AbstractThis protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. Curr. Protoc. Microbiol. 9:A.3D.1‐A.3D.6. © 2008 by John Wiley & Sons, Inc.
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Colony PCR

Cold Spring Harbor Protocols, 2006
Gina L, Costa, Michael P, Weiner
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Analyzing real-time PCR data by the comparative CT method

Nature Protocols, 2008
Thomas D. Schmittgen, K. Livak
semanticscholar   +1 more source

Competitive PCR

Nature, 1992
P D, Siebert, J W, Larrick
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Long PCR

Nature, 1994
S, Cheng   +3 more
openaire   +2 more sources

Additional modules for versatile and economical PCR‐based gene deletion and modification in Saccharomyces cerevisiae

Yeast, 1998
M. Longtine   +7 more
semanticscholar   +1 more source

Inverse PCR

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

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