Results 321 to 330 of about 3,963,364 (363)
Some of the next articles are maybe not open access.
The polymerase chain reaction (PCR) and RT-PCR
1997The polymerase chain reaction (PCR), a method for the in vitro enzymatic amplification of DNA, has facilitated greatly the analysis of complex genomes by permitting the characterization and cloning of DNA sequences from small samples of DNA or RNA of high sequence complexity. PCR and its specialized variants are now used as widely as any of the ‘older’
ARCA', Bruno, C. Savakis
openaire +3 more sources
PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification
2015The field of molecular biology was revolutionized with the development of the polymerase chain reaction (PCR). This chapter defines PCR, reverse transcription PCR (RT-PCR), real-time PCR, digital PCR and isothermal amplification. Within each subject a brief overview of the process is given along with the required reagents or components and highlighted ...
Rachel A. Bartholomew +3 more
openaire +1 more source
Enzymatic Amplification of RNA by PCR (RT‐PCR)
Current Protocols in Molecular Biology, 2001AbstractMethods for enzymatic amplification of RNA by the polymerase chain reaction (RT‐PCR) are highlighted in this unit. The is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery.
openaire +2 more sources
CODEHOP PCR and CODEHOP PCR Primer Design
2010While PCR primer design for the amplification of known sequences is usually quite straightforward, the design, and successful application of primers aimed at the detection of as yet unknown genes is often not. The search for genes that are presumed to be distantly related to a known gene sequence, such as homologous genes in different species, paralogs
Jeannette P, Staheli +3 more
openaire +2 more sources
2010
Alu PCR is a rapid and easy-to-perform "DNA fingerprinting" technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes.
openaire +2 more sources
Alu PCR is a rapid and easy-to-perform "DNA fingerprinting" technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes.
openaire +2 more sources
Direct PCR of Intact Bacteria (Colony PCR)
Current Protocols in Microbiology, 2008AbstractThis protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. Curr. Protoc. Microbiol. 9:A.3D.1‐A.3D.6. © 2008 by John Wiley & Sons, Inc.
openaire +2 more sources