Results 311 to 320 of about 3,963,364 (363)
Some of the next articles are maybe not open access.
cclm, 2000
Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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2003
Nonculture diagnosis is of increasing importance in maximizing case ascertainment of disease owing to Neisseria meningitidis (1). In the United Kingdom (UK), greater use of pre-admission antibiotics has lead to a steady decline in the total number of cases confirmed by culture, compared to the number reported to the Office for National Statistics (ONS).
M, Guiver, R, Borrow
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Nonculture diagnosis is of increasing importance in maximizing case ascertainment of disease owing to Neisseria meningitidis (1). In the United Kingdom (UK), greater use of pre-admission antibiotics has lead to a steady decline in the total number of cases confirmed by culture, compared to the number reported to the Office for National Statistics (ONS).
M, Guiver, R, Borrow
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2016
Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
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Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
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2017
Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
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Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
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The impact of the PCR plateau phase on quantitative PCR
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994The quantitative use of the polymerase chain reaction (PCR) is often compromised by the variability of the amplification. The most useful system for quantitation by PCR involves the use of controls which are almost identical to the target and which can be amplified using the same primers as the sequences of interest.
C, Morrison, F, Gannon
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2017
Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor, Johny +1 more
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Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor, Johny +1 more
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Direct PCR of Intact Bacteria (Colony PCR)
Current Protocols in Microbiology, 2016Abstract This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
Michael E, Woodman +3 more
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2010
Asynchronous PCR (aPCR) is a new PCR method that directs an ordered and sequential amplification of the + and - strands of DNA amplicons. There are several unique characteristics of aPCR that generate new application opportunities. The melting temperature (Tm) of the forward and reverse aPCR primers differ by at least 15°C.
Caifu, Chen, David, Ruff, Jason, Halsey
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Asynchronous PCR (aPCR) is a new PCR method that directs an ordered and sequential amplification of the + and - strands of DNA amplicons. There are several unique characteristics of aPCR that generate new application opportunities. The melting temperature (Tm) of the forward and reverse aPCR primers differ by at least 15°C.
Caifu, Chen, David, Ruff, Jason, Halsey
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2013
The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it can be adequately detected, often by agarose gel electrophoresis. PCR is routinely used to amplify, modify, and clone genes for expression studies.
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The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it can be adequately detected, often by agarose gel electrophoresis. PCR is routinely used to amplify, modify, and clone genes for expression studies.
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2003
Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
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Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
openaire +2 more sources