Results 321 to 330 of about 3,284,156 (346)
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2003
Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
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Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
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Analyzing real-time PCR data by the comparative CT method
Nature Protocols, 2008Thomas D. Schmittgen, K. Livak
semanticscholar +1 more source
2010
Alu PCR is a rapid and easy-to-perform "DNA fingerprinting" technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes.
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Alu PCR is a rapid and easy-to-perform "DNA fingerprinting" technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes.
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2003
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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An economic method for the fluorescent labeling of PCR fragments
Nature Biotechnology, 2000M. Schuelke
semanticscholar +1 more source