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Current Protocols Essential Laboratory Techniques, 2008
AbstractThe polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique ...
Christine D. Kuslich +2 more
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AbstractThe polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique ...
Christine D. Kuslich +2 more
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Water Research, 2005
We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Thaís Cristine Marques Sincero +3 more
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We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Thaís Cristine Marques Sincero +3 more
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Analytical and Bioanalytical Chemistry, 2003
Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable
L J, Kricka, P, Wilding
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Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable
L J, Kricka, P, Wilding
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CODEHOP PCR and CODEHOP PCR Primer Design
2010While PCR primer design for the amplification of known sequences is usually quite straightforward, the design, and successful application of primers aimed at the detection of as yet unknown genes is often not. The search for genes that are presumed to be distantly related to a known gene sequence, such as homologous genes in different species, paralogs
Timothy M. Rose +4 more
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2016
Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
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Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
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PCR in Meningoencephalitis Diagnosis
Scandinavian Journal of Infectious Diseases, 2000Polymerase chain reaction (PCR) detection of a stretch of nucleic acid sequence of microbial origin from a clinical sample is not always diagnostic of disease unless the identified agent is a strict pathogen or its growth is documented. We describe here a case of acute meningoencephalitis in a 21-y-old man, in whom no pathogen was isolated by ...
SGARABOTTO D +6 more
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Nanoparticle PCR: Nanogold‐Assisted PCR with Enhanced Specificity
Angewandte Chemie International Edition, 2005Shanghai Jiao Tong Univ, BioX DNA Comp Consortium, Shanghai 200030, Peoples R China; Shanghai Jiao Tong Univ, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China; Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R ...
Jun Hu +9 more
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2003
Nonculture diagnosis is of increasing importance in maximizing case ascertainment of disease owing to Neisseria meningitidis (1). In the United Kingdom (UK), greater use of pre-admission antibiotics has lead to a steady decline in the total number of cases confirmed by culture, compared to the number reported to the Office for National Statistics (ONS).
M, Guiver, R, Borrow
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Nonculture diagnosis is of increasing importance in maximizing case ascertainment of disease owing to Neisseria meningitidis (1). In the United Kingdom (UK), greater use of pre-admission antibiotics has lead to a steady decline in the total number of cases confirmed by culture, compared to the number reported to the Office for National Statistics (ONS).
M, Guiver, R, Borrow
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2010
Asynchronous PCR (aPCR) is a new PCR method that directs an ordered and sequential amplification of the + and - strands of DNA amplicons. There are several unique characteristics of aPCR that generate new application opportunities. The melting temperature (Tm) of the forward and reverse aPCR primers differ by at least 15°C.
Caifu, Chen, David, Ruff, Jason, Halsey
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Asynchronous PCR (aPCR) is a new PCR method that directs an ordered and sequential amplification of the + and - strands of DNA amplicons. There are several unique characteristics of aPCR that generate new application opportunities. The melting temperature (Tm) of the forward and reverse aPCR primers differ by at least 15°C.
Caifu, Chen, David, Ruff, Jason, Halsey
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2003
Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
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Quantitative PCR recently has become a powerful tool in clinical investigations. Its main areas of application have been the assessment of residual disease after treatment of leukemia and lymphoma, the detection of viral nucleic acids, and the detection of gene amplification or deletion and of aneuploidy (1).The application of PCR as a quantitative ...
openaire +2 more sources