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A streamlined POCT solution for rapid infectious disease detection. [PDF]
Sci RepXie Y +8 more
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Protocol for the functional evaluation of genetic variants using saturation genome editing. [PDF]
STAR ProtocObolenski S +4 more
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A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger. [PDF]
Fungal Biol Biotechnolvan Esch AP +5 more
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European Journal of Cancer, 1992
cytotoxicity in human ovarian cancer cell lines by glutathione depletion. Cancer Res 1984,44,5427-5431. 51. Hansson J, Berhane K, Castro VM, Jungnelius U, Mannervik B, Ringborg U. Sensitization of human melanoma cells to the cytotoxic effects of melphalan by the glutathione transferase inhibitor e-&crynic acid. CancerRes 1991,51,94-98. 52.
Alasdair C. Stamps +2 more
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cytotoxicity in human ovarian cancer cell lines by glutathione depletion. Cancer Res 1984,44,5427-5431. 51. Hansson J, Berhane K, Castro VM, Jungnelius U, Mannervik B, Ringborg U. Sensitization of human melanoma cells to the cytotoxic effects of melphalan by the glutathione transferase inhibitor e-&crynic acid. CancerRes 1991,51,94-98. 52.
Alasdair C. Stamps +2 more
openaire +3 more sources
The polymerase chain reaction (PCR) and RT-PCR
1997The polymerase chain reaction (PCR), a method for the in vitro enzymatic amplification of DNA, has facilitated greatly the analysis of complex genomes by permitting the characterization and cloning of DNA sequences from small samples of DNA or RNA of high sequence complexity. PCR and its specialized variants are now used as widely as any of the ‘older’
ARCA', Bruno, C. Savakis
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2017
Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor Johny, Sarita G. Bhat
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Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor Johny, Sarita G. Bhat
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Water Research, 2005
We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Thaís Cristine Marques Sincero +3 more
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We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Thaís Cristine Marques Sincero +3 more
openaire +3 more sources