Results 281 to 290 of about 410,073 (330)

Gene Amplification and qRT-PCR

2014
This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Alain Filloux, Cerith Jones
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PCR Amplification of Base‐Modified DNA

Current Protocols in Chemical Biology, 2018
AbstractAn efficient PCR amplification of various templates (short 57‐mer, random 67‐ and 82‐mer, and long DNA) with base‐modified nucleoside triphosphates is presented here. Using 5‐substituted pyrimidine and 7‐substituted‐7‐deaza‐ or 8‐substituted purine nucleoside triphosphates as substrates for thermostable DNA polymerases [Taq and Vent (exo ...
Eremeeva, Elena, Herdewijn, Piet
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PCR Amplification of DNA

Journal of Chemical Education, 1994
A polymerase chain reaction (PCR) student exercise using a teacup thermocycler and a commercially available thermocycler.
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PCR amplification reactions in parasitology

Journal of Microbiological Methods, 1995
The development of PCR systems for the detection and identification of pathogenic human parasites has only recently started. Evaluation of most of these methods is still lacking. Most likely these methods will need considerable improvement before reliable diagnostic methods become available.
Henk L. Smits, Rudy A. Hartskeerl
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Amplification of DNA and RNA by PCR [PDF]

, 2003
The polymerase chain reaction (PCR) has revolutionized many areas of medicine, including hemostasis. Although this volume is not devoted to PCR, many of the chapters employ the technique at some point to amplify specific DNA or RNA sequences. This chapter, therefore, provides a brief outline of the techniques and methods for the amplification of both ...
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PCR microfluidic devices for DNA amplification

Biotechnology Advances, 2006
The miniaturization of biological and chemical analytical devices by micro-electro-mechanical-systems (MEMS) technology has posed a vital influence on such fields as medical diagnostics, microbial detection and other bio-analysis. Among many miniaturized analytical devices, the polymerase chain reaction (PCR) microchip/microdevices are studied ...
Chunsun Zhang, Jinliang Xu, Wen-Ling Zheng, Wen-Li Ma   +3 more
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Enzymatic Amplification of RNA by PCR (RT‐PCR)

Current Protocols in Molecular Biology, 2001
AbstractMethods for enzymatic amplification of RNA by the polymerase chain reaction (RT‐PCR) are highlighted in this unit. The is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery.
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Statistical Estimations of PCR Amplification Rates

1998
Quantitative applications of the Polymerase Chain Reaction (PCR), also known as Quantitative-PCR (Q-PCR) are intended either to determine the number of copies of a given nucleic acid sequence, or more generally, to determine the relative abundance of two sequences.
Peccoud, Jean, Jacob, C.
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PCR amplification of antibody genes

Methods, 1991
A general method for directly obtaining the DNA sequence of the variable regions of any immunoglobulin chain by using a mixture of oligomer primers and the polymerase chain reaction (PCR) is described. Mixed oligonucleotide primers corresponding to the 5′ signal peptide or framework 1 and a conserved 3′ constant region primer were used for enzymatic ...
James W. Larrick, Kirk E. Fry
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Biochip for PCR amplification in silicon

1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451), 2002
A micro liter PCR (polymerase chain reaction) biochip was designed and fabricated for DNA amplification. It was consist of a vessel, platinum thin film heater and a temperature sensor. The heater and sensor were deposited on to the bottom of the vessel. Its advantage is rapid thermal cycling time constant.
C. Dafu, W. Li, Z. Zhan, Y. Zhongyao
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