Results 291 to 300 of about 410,073 (330)

Multiplex PCR Amplification of Ancient DNA

2011
Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Tara L. Fulton, Mathias Stiller
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Amplification of Genomic DNA by PCR

2003
The polymerase chain reaction (PCR) is used to amplify a segment of DNA that lies between two regions of known sequence (1-3). It requires two oligonucleotide primers that flank the DNA fragment to be amplified and employs repeated cycles of heat denaturation of the DNA, annealing of the primers to their complementary sequences, and extension of the ...
openaire   +3 more sources

PCR-mediated whole genome amplification of phytoplasmas

Journal of Microbiological Methods, 2004
A method was developed for genome analysis of phytoplasmas, bacterial plant pathogens that cannot be cultivated in vitro in cell-free media. The procedure includes a CsCl-bisbenzimide gradient buoyant centrifugation followed by polymerase chain reaction (PCR)-mediated whole genome amplification.
GARCIA CHAPA M., A. BATLLE, REKAB, Djaouida, RUIZ ROSQUETE M., FIRRAO, Giuseppe   +4 more
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Non-PCR Amplification Techniques

2018
Signal, probe, and non-PCR target amplification methods have been developed as viable alternatives to PCR testing for infectious agents. These signal amplification methods include hybrid capture and Invader, as well as target amplification methods such as transcription-mediated amplification, strand displacement amplification, loop-mediated isothermal ...
Elizabeth M. Marlowe, Rosemary C. She, Ted E. Schutzbank   +2 more
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PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification

2015
The field of molecular biology was revolutionized with the development of the polymerase chain reaction (PCR). This chapter defines PCR, reverse transcription PCR (RT-PCR), real-time PCR, digital PCR and isothermal amplification. Within each subject a brief overview of the process is given along with the required reagents or components and highlighted ...
Janine R. Hutchison, Rachel A. Bartholomew, Douglas R. Call, Timothy M. Straub   +3 more
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Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

2012
Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in ...
Foissac, Xavier, Danet, Jean-Luc, Malembic-Maher, Sylvie, Salar, Pascal, Safarova, Dana, Valova, Pavla, Navratil, Milan   +6 more
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Closed chamber PCR-chips for DNA amplification

IEE Seminar on Demonstrated Micromachining Technologies for Industry, 2000
The polymerase chain reaction (PCR) is a well described method for the selective identical replication of DNA molecules. By an enzymatic in-vitro amplification process, the concentration of a DNA species is nearly doubled in a process, stepping through three different temperatures.
A Brunnschweiler, M A Lee, Alan G. R. Evans, D L Leslie, C G J Schabmueller, G. Ensell   +5 more
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Virus Testing by PCR and RT-PCR Amplification in Berry Fruit

2015
Berry fruit crops are prone to infection by a wide range of viruses, with the list expanding every year, primarily because of the expansion of the crops to new geographic regions. Although some methods allow for virus detection in a nonspecific manner, the advent of cheap and effective nucleic acid sequencing technologies has allowed for the ...
Stuart A. MacFarlane, Wendy J. McGavin, Ioannis E. Tzanetakis   +2 more
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The Limit of PCR Amplification

Journal of Theoretical Biology, 1996
Mariana A. Apostolova, Dimiter S. Dimitrov   +1 more
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PCR amplification of insect ribosomal DNA

1997
Ribosomal DNA (rDNA) has been used extensively and very effectively for insect phylogenetic and population analysis. In this chapter, we will focus on its use in distinguishing between closely-related species. The rDNA genes encode the rRNA, which comprises three of the main RNA structural components of ribosomes. Because rRNA has a fundamental role in
Frank H. Collins, Susan M. Paskewitz
openaire   +2 more sources