Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes [PDF]
Archives of Biological Sciences, 2012 The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1 ...
Trifunović Milana +6 more
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Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
, 1992 Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson +15 more
core +1 more source
Cogent Food & Agriculture, 2018 Extraction of high quality DNA is crucial for any molecular genetic analysis. However, it is difficult to be obtained from problematic plant tissue, high in phenolic compounds, such as apple leaves.
Barbara Pipan +4 more
doaj +1 more source
Cloning, expression, purification and characterization of Leishmania tropica PDI-2 protein
Open Life Sciences, 2016 In Leishmania species, protein disulfide isomerase (PDI) is an essential enzyme that catalyzes thiol-disulfide interchange. The present work describes the isolation, cloning, sequencing and expression of the pdI-2 gene.
Ali Dina +3 more
doaj +1 more source
Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF) [PDF]
, 2013 Purpose: The objective of this study was construction of recombinant hEGF-pPIC9 which may be used for expression of recombinant hEGF in following studies. Methods: EGF cDNA was purchased from Genecopoeia Company and used for PCR amplification.
Jamal Mohammadian +3 more
core +1 more source
Whole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR) [PDF]
Cold Spring Harbor Protocols, 2008 INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias.
Nona, Arneson +3 more
openaire +2 more sources
Interference in PCR: Transcription amplifications of mixed PVY isolates
Plant Protection Science, 2005 Interference in PCR and transcription amplifications by mixed virus templates is described. The yields of PCR amplification of closely related PVY isolates in a mixture were lower than those of the separate amplification of each individual virus strain ...
Arie Rosner, Ludmila Maslenin
doaj +1 more source
Micelle PCR reduces chimera formation in 16S rRNA profiling of complex microbial DNA mixtures [PDF]
, 2015 16S rRNA gene profiling has revolutionized the field of microbial ecology. Many researchers in various fields have embraced this technology to investigate bacterial compositions of samples derived from many different ecosystems.
Boers, S.A. (Stefan) +2 more
core +3 more sources
Examining peak height ratios in low template DNA samples with and without sampling using a single-tube extraction protocol [PDF]
, 2015 The developments of the polymerase chain reaction (PCR) and the short tandem repeat multiplex kits increased the ease and lowered the time and sample quantity required for deoxyribonucleic acid (DNA) typing compared to previous methods.
Nguyen, Thutrang Thi
core +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source