Results 31 to 40 of about 709,414 (333)
Cogent Food & Agriculture, 2018 Extraction of high quality DNA is crucial for any molecular genetic analysis. However, it is difficult to be obtained from problematic plant tissue, high in phenolic compounds, such as apple leaves.
Barbara Pipan +4 more
doaj +1 more source
BioTechniques, 1998 Previously we described a PCR protocol for detecting the inversion in the factor VIII gene, which is a common cause of Hemophilia A. This PCR assay is challenging due to the size of the amplification (10–12 kb), the varying GC content (30%–80%) and the ...
Qiang Liu, Steve S. Sommer
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‘Next‐base’ effect on PCR amplification
Environmental Microbiology Reports, 2011 SummaryThe base adjacent to the 3′ end of universal PCR primers targeting the 16S rRNA gene is often variable and apparently biases the microbial community composition as represented by PCR‐based surveys. To test this hypothesis, four templates of 44 bases each and two complementary primers (21 bases) were designed to differ only in the bases adjacent ...
Ariel Kushmaro +4 more
openaire +5 more sources
Counting unique molecular identifiers in sequencing using a multitype branching process with immigration [PDF]
arXiv, 2022 Detection of extremely rare variant alleles, such as tumour DNA, within a complex mixture of DNA molecules is experimentally challenging due to sequencing errors. Barcoding of target DNA molecules in library construction for next-generation sequencing provides a way to identify and bioinformatically remove polymerase induced errors.
arxiv
Interference in PCR: Transcription amplifications of mixed PVY isolates
Plant Protection Science, 2005 Interference in PCR and transcription amplifications by mixed virus templates is described. The yields of PCR amplification of closely related PVY isolates in a mixture were lower than those of the separate amplification of each individual virus strain ...
Arie Rosner, Ludmila Maslenin
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DMSO Improves the Ski-Slope Effect in Direct PCR
Applied Sciences, 2021 Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA ...
Joo-Young Kim +6 more
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Stochastics of DNA Quantification [PDF]
arXiv, 2023 A common approach to quantifying DNA involves repeated cycles of DNA amplification. This approach, employed by the polymerase chain reaction (PCR), produces outputs that are corrupted by amplification noise, making it challenging to accurately back-calculate the amount of input DNA.
arxiv
Dynamics and Control of DNA Sequence Amplification [PDF]
, 2014 DNA amplification is the process of replication of a specified DNA sequence \emph{in vitro} through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first ...
arxiv +1 more source
Formalin fixation at low temperature provides better yield and integrity of DNA [PDF]
Medicinski Podmladak, 2019 Introduction: Fixation with formalin, a widely used procedure to preserve tissue samples, leads to damage of DNA through crosslinking activity. The factors that could influence the fixation and integrity of DNA may be numerous: incubation time, tissue ...
Makulović Stefan +4 more
doaj
Scientific Reports, 2021 The development of alternative isothermal amplification assays including multiple cross displacement amplification (MCDA) may address speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection.
Laurence Don Wai Luu +4 more
doaj +1 more source