Results 71 to 80 of about 1,169,901 (341)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Nucleic acid amplification: Alternative methods of polymerase chain reaction

Journal of Pharmacy and Bioallied Sciences, 2013
Nucleic acid amplification is a valuable molecular tool not only in basic research but also in application oriented fields, such as clinical medicine development, infectious diseases diagnosis, gene cloning and industrial quality control.
Md Fakruddin, Khanjada Shahnewaj Bin Mannan, Abhijit Chowdhury, Reaz Mohammad Mazumdar, Md Nur Hossain, Sumaiya Islam, Md Alimuddin Chowdhury   +6 more
doaj   +1 more source

Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]

, 1994
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Blonden, Lau, Cremer, Marion, Cremer, Thomas, Kilian, Karin, Ried, Thomas, Schröck, Evelin, Tocharoentanaphol, Chintana   +6 more
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations [PDF]

, 2017
© 2017 Sellers et al. The tadpole shrimp, Triops cancriformis, is a freshwater crustacean listed as endangered in the UK and Europe living in ephemeral pools.
Griffin, Larry R., Gómez, Africa, Hänfling, Bernd, Sellers, Graham S.   +3 more
core   +3 more sources

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

Strand Displacement Chain Reaction (SDCR): New Hybrid Amplification Technique for Fast and Sensitive Detection of Genetic Materials

Biomolecules
Nucleic acid amplification methods are widely used in science, medicine and forensics for molecular biological assays and for the detection of genetic material.
Evgeniya V. Smirnova, Ekaterina V. Barsova, Dmitriy A. Varlamov, Vladimir M. Kramarov, Konstantin A. Blagodatskikh, Konstantin B. Ignatov   +5 more
doaj   +1 more source

Rapid Multiplexed Reduced Representation Bisulfite Sequencing Library Prep (rRRBS)

Bio-Protocol, 2019
DNA methylation is a common mechanism of epigenetic regulation involved in transcriptional modulation and genome stability. With the evolution of next-generation sequencing technologies, establishing quantitative genome-wide DNA methylation profiles is ...
Lisa-Marie Legault, Donovan Chan, Serge McGraw   +2 more
doaj   +1 more source

Strangles: The Molecular Identification and Epidemiology of Streptococcus equi subsp. equi [PDF]

, 2012
A conventional PCR diagnostic test was established to confirm the microbiological isolation of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles.
Patty, Olivia Anne
core   +1 more source

An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans

, 2019
The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential.
Aguayo, F., Contreras, R., Guggiana-Nilo, D., Porcile, V.   +3 more
core   +1 more source