Results 11 to 20 of about 173,457,123 (221)

PCR-based methods to the diagnosis of imported malaria [PDF]

Parasite, 2008
Rapid and precise diagnosis of malaria is needed to take care febrile patient returning from endemic areas. Since the first description of the diagnosis of Plasmodium infection by polymerase-chain-reaction (PCR), the role of this kind of molecular method
Berry A., Benoit-Vical F., Fabre R., Cassaing S., Magnaval J.F.   +4 more
doaj   +5 more sources

PCR as a Confirmatory Technique for Laboratory Diagnosis of Malaria [PDF]

Journal of Clinical Microbiology, 2006
ABSTRACT We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.
Stephanie P, Johnston, Norman J, Pieniazek, Maniphet V, Xayavong, Susan B, Slemenda, Patricia P, Wilkins, Alexandre J, da Silva   +5 more
openaire   +2 more sources

Comparison of the OptiMAL Test with PCR for Diagnosis of Malaria in Immigrants [PDF]

Journal of Clinical Microbiology, 1999
ABSTRACT The OptiMAL test (Flow Inc., Portland, Oreg.), which detects a malaria parasite lactate dehydrogenase (pLDH) antigen, has not been evaluated for its sensitivity in the diagnosis of malaria infection in various epidemiological settings.
J, Iqbal, A, Sher, P R, Hira, R, Al-Owaish   +3 more
openaire   +2 more sources

Comparative analysis of four malaria diagnostic tools and implications for malaria treatment in southwestern Nigeria

International Journal of Infectious Diseases, 2021
Objectives: One of the problems encountered in malaria control and elimination is inaccurate diagnosis, resulting from the degree of sensitivity of the different malaria diagnostic tools.
Mary Aigbiremo Oboh, Eniyou Cheryl Oriero, Tolla Ndiaye, Aida Sadikh Badiane, Daouda Ndiaye, Alfred Amambua-Ngwa   +5 more
doaj   +1 more source

Investigation of Sensitivity of Rapid Diagnosis Tests in Patients with Suspected Malaria

Türkiye Parazitoloji Dergisi
Objective: Malaria has been eradicated in Türkiye as of 2010, but there are imported cases. In this study, we aimed to compare the diagnostic value of two rapid tests; SD Bioline Malaria Ag Pf/Pan (SD-Pf/Pan) and SD Bioline Malaria Ag Pf/Pv (SD-Pf/Pv ...
Gülnaz Çulha, Yusuf Önlen, Mehmet Çabalak, Tuğba Kaya, Burcu Küçükeser   +4 more
doaj   +2 more sources

A Rapid FRET Real-Time PCR Protocol for Simultaneous Quantitative Detection and Discrimination of Human Plasmodium Parasites

Bio-Protocol, 2022
Malaria is the most important parasitic disease worldwide, and accurate diagnosis and treatment without delay are essential for reducing morbidity and mortality, especially in P. falciparum malaria.
Renate Schneider, Aline Lamien-Meda, Herbert Auer, Ursula Wiedermann-Schmidt, Peter Chiodini, Julia Walochnik   +5 more
doaj   +1 more source

Evaluation of the RealArt Malaria LC Real-Time PCR Assay for Malaria Diagnosis [PDF]

Journal of Clinical Microbiology, 2004
ABSTRACT PCR-based methods have advantages over traditional microscopic methods for the diagnosis of malaria, especially in cases of low parasitemia and mixed infections. However, current PCR-based assays are often labor-intensive and not readily quantifiable and have the potential for contamination due to a requirement for postamplification ...
Gabriella A, Farcas, Kathleen J Y, Zhong, Tony, Mazzulli, Kevin C, Kain   +3 more
openaire   +2 more sources

Performance Evaluation of Nested Polymerase Chain Reaction (Nested PCR), Light Microscopy, and Plasmodium falciparum Histidine-Rich Protein 2 Rapid Diagnostic Test (PfHRP2 RDT) in the Detection of Falciparum Malaria in a High-Transmission Setting in Southwestern Nigeria

Pathogens, 2022
Malaria remains a major public health challenge worldwide. In order to ensure a prompt and accurate malaria diagnosis, the World Health Organization recommended the confirmatory parasitological diagnosis of malaria by microscopy and malaria rapid ...
Oluwaseun Bunmi Awosolu, Zary Shariman Yahaya, Meor Termizi Farah Haziqah, Titus Adeniyi Olusi   +3 more
doaj   +1 more source

PCR - based diagnosis to evaluate the performance of malaria reference centers [PDF]

Revista do Instituto de Medicina Tropical de São Paulo, 2004
Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P.
Di Santi, Silvia Maria, Kirchgatter, Karin, Brunialti, Karen Cristina Sant'Anna, Oliveira, Alessandra Mota, Ferreira, Sergio Roberto Santos, Boulos, Marcos   +5 more
openaire   +4 more sources

Evaluating performance of multiplex real time PCR for the diagnosis of malaria at elimination targeted low transmission settings of Ethiopia

Malaria Journal, 2022
Background Malaria incidence has declined in Ethiopia in the past 10 years. Current malaria diagnostic tests, including light microscopy and rapid antigen-detecting diagnostic tests (RDTs) cannot reliably detect low-density infections. Studies have shown
Mahlet Belachew, Mistire Wolde, Desalegn Nega, Bokretsion Gidey, Legessie Negash, Ashenafi Assefa, Geremew Tasew, Adugna Woyessa, Adugna Abera   +8 more
doaj   +1 more source