Results 31 to 40 of about 173,457,123 (221)

Comparison of detection methods to estimate asexual Plasmodium falciparum parasite prevalence and gametocyte carriage in a community survey in Tanzania [PDF]

, 2014
The use of molecular techniques to detect malaria parasites has been advocated to improve the accuracy of parasite prevalence estimates, especially in moderate to low endemic settings. Molecular work is time-consuming and costly, thus the effective gains
Kabanywanyi, Abdu-Noor M., Mwingira, Felista, Felger, Ingrid, Genton, Blaise, Felista Mwingira, Mwingira F, Ingrid Felger, Genton B, Abdu-Noor M Kabanywanyi, Blaise Genton, Felger I, Kabanywanyi AN   +11 more
core   +1 more source

Over-diagnosis of malaria is not a lost cause. [PDF]

, 2006
BACKGROUND: Recent studies have highlighted the over-diagnosis of malaria in clinical settings in Africa. This study assessed the impact of a training programme implemented as part of an intervention trial on diagnostic behaviour of clinicians in a rural
Urassa Raymond, Jackline Mosha, Daniel Chandramohan, Semarundu Waziri J, Masika, Paul M, Masika Paul M, Gosling, Roly D, Semarundu, Waziri J, Mosha, Jackline, Paul M Masika, Chandramohan, Daniel, Gosling Roly D, Waziri J Semarundu, Urassa, Raymond, Roly D Gosling, Chandramohan Daniel, Mosha Jackline, Raymond Urassa   +17 more
core   +1 more source

Considerations on PCR-based methods for malaria diagnosis in China malaria diagnosis reference laboratory network

BioScience Trends, 2018
Precise diagnosis is a key measurement for malaria control and elimination, traditional microscopy and rapid diagnostic tests cannot satisfy the requirements especially in the low transmission endemic areas or in the malaria elimination phase. Polymerase chain reaction (PCR) with high sensitivity and specificity can be considered as a diagnostic ...
Yin, Jianhai, Li, Mei, Yan, He, Zhou, Shuisen   +3 more
openaire   +3 more sources

Malaria diagnosis from pooled blood samples: comparative analysis of real-time PCR, nested PCR and immunoassay as a platform for the molecular and serological diagnosis of malaria on a large-scale [PDF]

Memórias do Instituto Oswaldo Cruz, 2011
Malaria diagnoses has traditionally been made using thick blood smears, but more sensitive and faster techniques are required to process large numbers of samples in clinical and epidemiological studies and in blood donor screening. Here, we evaluated molecular and serological tools to build a screening platform for pooled samples aimed at reducing both
Lima, Giselle, Levi, José, Geraldi, Marcelo, Sanchez, Maria, Segurado, Aluísio, Hristov, Angélica, Inoue, Juliana, Costa-Nascimento, Maria, Silvia, Di Santi   +8 more
openaire   +4 more sources

Diagnostic performance of CareStart™ malaria HRP2/pLDH (Pf/pan) combo test versus standard microscopy on falciparum and vivax malaria between China-Myanmar endemic borders

Malaria Journal, 2013
Background Rapid diagnostic test (RDT) is becoming an alternative way of establishing quickly the diagnosis of malaria infections, by detecting specific malaria antigens in suspected patients’ blood between the China-Myanmar endemic borders areas ...
Xiaodong Sun, Tambo Ernest, Chun Wei, Zhibin Cheng, Yan Deng, Jian Wang, Jiazhi Wang, Xiaonong Zhou   +7 more
doaj   +1 more source

Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia

Acta Protozoologica, 2023
Malaria diagnosis continues to be one of the most important steps in the cycle of control specially in endemic countries with low parasitic load infections.
Rasha H. Solimam, Alexandra Martin-Ramirez, Jose M. Rubio, Eman A. Khalifa, Bushra E. Hussein, Maha M. Wahab, Marta Lanza, Yousry A. Hawash   +7 more
doaj   +1 more source

Loop-Mediated Isothermal PCR (LAMP) for the Diagnosis of Falciparum Malaria

The American Journal of Tropical Medicine and Hygiene, 2007
A recently described loop-mediated isothermal polymerase chain reaction (LAMP) for molecular detection of Plasmodium falciparum was compared with microscopy, PfHRP2-based rapid diagnostic test (RDT), and nested polymerase chain reaction (PCR) as the “gold standard” in 115 Bangladeshi in-patients with fever.
Paris, D, Imwong, M, Faiz, M, Hasan, M, Bin Yunus, E, Silamut, K, Lee, S, Day, N, Dondorp, A   +8 more
openaire   +4 more sources

Diagnosis and treatment based on quantitative PCR after controlled human malaria infection [PDF]

Malaria Journal, 2016
Controlled human malaria infection (CHMI) has become well-established in the evaluation of drugs and vaccines. Anti-malarial treatment is usually initiated when thick blood smears are positive by microscopy. This study explores the effects of using the more sensitive qPCR as the primary diagnostic test.1691 diagnostic blood samples were analysed by ...
Walk, J., Schats, R., Langenberg, M.C., Reuling, I.J., Teelen, K., Roestenberg, M., Hermsen, C.C., Visser, L.G., Sauerwein, R.W.   +8 more
openaire   +5 more sources

Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?

Memorias do Instituto Oswaldo Cruz, 2014
The polymerase chain reaction (PCR)-based methods for the diagnosis of malaria infection are expected to accurately identify submicroscopic parasite carriers.
Daniela Camargos Costa, Ana Paula Madureira, Lara Cotta Amaral, Bruno Antonio Marinho Sanchez, Luciano Teixeira Gomes, Cor Jesus Fernandes Fontes, Jean Ezequiel Limongi, Cristiana Ferreira Alves de Brito, Luzia Helena Carvalho   +8 more
doaj   +1 more source

Comparative Diagnosis of Malaria Infections by Microscopy, Nested PCR, and LAMP in Northern Thailand [PDF]

The American Society of Tropical Medicine and Hygiene, 2010
Three methods, microscopy, nested polymerase chain reaction (nPCR), and loop-mediated isothermal amplification (LAMP) have been applied for malaria diagnosis in 105 human blood samples collected in Northern Thailand. Only Plasmodium falciparum and Plasmodium vivax
Birgit, Pöschl, Jarurin, Waneesorn, Oriel, Thekisoe, Salakchit, Chutipongvivate, Panagiotis, Karanis, Karanis, Panagiotis   +5 more
openaire   +2 more sources