Results 71 to 80 of about 1,870 (176)

Development and Application of a Rep‐Targeted SYBR Green I qPCR Assay for Porcine Circovirus 4: Etiological Investigation in China (2022–2025) and Molecular Evolutionary Analysis

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Porcine circovirus (PCV) type 4 (PCV4) is an emerging Circovirus whose epidemiological and evolutionary dynamics remain incompletely characterized. We developed a replication‐associated protein (Rep)‐targeted SYBR Green I real‐time quantitative PCR assay that showed analytical specificity against tested common porcine pathogens and an estimated 95 ...
Yi Lu, Yanhong Chen, Tong Xu, Yang Zeng, Ling Dong, Zhijing Mei, Xuexiang Yu, Suphot Wattanaphansak, Wentao Li, Qigai He, Zongfu Wu   +10 more
wiley   +1 more source

Establishment and Application of a Novel Protein Microarray for Serological Detection and Differentiation of Senecavirus A

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Senecavirus A (SVA) is an emerging swine pathogen that causes vesicular disease, which presents clinically indistinguishable signs from other vesicular diseases. To enable differentiation of infected from vaccinated animals (DIVA), we developed a novel protein microarray for dual serological detection of antibodies against SVA structural (VP2‐VP3‐VP1 ...
Dexin Li, Junhua Deng, Zenglin Wang, Yunjing Zhang, Yufang Li, Liying Hao, Kegong Tian, Xiangdong Li, Linzhu Ren   +8 more
wiley   +1 more source

Recent Advances in Lateral Flow Immunoassay for Rapid Diagnosis of Viral Diseases

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Viral diseases are a major threat to human and animal health, as illustrated by recent pandemics like COVID‐19 and African swine fever (ASF). Timely, accurate detection of viral infections is critical for effective disease control. Among diverse diagnostic techniques, lateral flow immunoassay (LFIA) has become a widely used on‐site testing tool, owing ...
Quanyu Ren, Yan Wang, Haoyuan Ma, Jialiang Xie, Jianyou Jin, Rumeng Tian, Hao Yu, Xu Gao, Nanhua Chen   +8 more
wiley   +1 more source

Isolation of porcine circovirus 3 using primary porcine bone marrow-derived cells

Virology Journal
Porcine circovirus 3 (PCV3) was first reported in the United States in 2016; this virus is considered to be involved in diverse pathologies, such as multisystem inflammation, porcine dermatitis and nephropathy syndrome, and reproductive disorders ...
Shizuka Hayashi, Fumihiko Katakura, Tadaaki Moritomo, Nobuyuki Tsutsumi, Katsuaki Sugiura, Tetsuo Sato   +5 more
doaj   +1 more source

Evidence of Spillover and Recombination Between Domestic Pigs and Wild Boars Provides New Insights into Porcine Circoviruses. [PDF]

Pathogens
Porcine circovirus types 2 (PCV2) and 3 (PCV3) are major pathogens affecting swine health and productivity, yet important gaps remain in understanding their evolution and circulation in Europe, particularly within wild boar populations that may serve as ...
Almeida B, Duarte MD, Duarte A, Barros SC, Abade Dos Santos F, Henriques AM.   +5 more
europepmc   +2 more sources

A Novel Attenuated NADC30‐Like PRRSV Vaccine Candidate Provides Cross‐Protection Against Homologous and Heterologous Viral Challenge

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) is one of the major pathogens threatening the global swine industry and causes enormous economic losses annually. Vaccination remains one of the most important strategies for the prevention and control of PRRSV infection.
Heng Zhang, Jiakai Zhao, Chengxin Zhang, Ting Tang, Qianyi Zhu, Hui Zhou, Yingru Ma, Xiaohong Qi, Qin Zhao, Yani Sun, Nan-hua Chen   +10 more
wiley   +1 more source

A Case report of reproductive failure and dermatitis occurred by PCV3 and Mycoplasma suis in sows at a breeding farm

Veterinary Integrative Sciences
Reproductive disorders in sows pose significant economic challenges to the swine industry worldwide. In this study, we investigated the presence of porcine circovirus type 3 (PCV3) and Mycoplasma suis (M.
Dung Thi Thuy Do, Tram Thi Ngoc Ngo, Nam Minh Nguyen, Nga Thi To Bui, Duy Tien Do   +4 more
doaj   +1 more source

High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

AMB Express, 2020
Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate.
Bing Yan Liu, Bin Gao, Meng Zhi Liu, Ting Ting Zhang, Bao Shan Liu, Ze Liang Chen   +5 more
doaj   +1 more source

The Quadruplex Real‐Time PCR Assay for Simultaneous Detection of GETV, PRRSV, JEV, and PCV2 in Swine

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
This study aimed to establish a quadruplex real‐time PCR assay for the simultaneous detection of Getah virus (GETV), Porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus (JEV), and Porcine circovirus type 2 (PCV2).
Haojie Wang, Lihong Xue, Bowen Shan, Shuyan Wu, Tongqin An, Changqing Yu, Changyou Xia, He Zhang, Taotao Yang   +8 more
wiley   +1 more source

Emergence of Escherichia coli Hybrid Pathotype ETEC/STEC: A Macroepidemiological Approach of Molecular Characterization in the United States Swine Population

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Escherichia coli–associated diseases continue to be a significant concern in swine health. Disease may occur when E. coli strains harbor specific virulence factors. The combination of virulence factors detected in a single isolate forms a virotype, which can be grouped into pathotypes based on mechanisms of pathogenicity. This study compiled E.
Elisa De Conti, Guilherme Cezar, Thuc Quyen Le, Meghana Korada, Kinath Rupasinghe, Hemant K. Naikare, Angela Pillatzki, Craig L. Bowen, Melanie Prarat, Franco Matias Ferreyra, Rodger Main, Paul Yeske, Mark Schwartz, Beth Thompson, Peter Schneider, Samuel Copeland, Luc Dufresne, Daniel Boykin, Megan Niederwerder, Deborah Murray, Brigitte Mason, Corrine Frugé, William Hollis, Rebecca Robbins, Thomas Petznick, Kurt Kuecker, Lauren Glowzenski, Brooke Kitting, Gustavo Silva, Bret Crim, Eric Burrough, Phillip Gauger, Michael Zeller, Sara McReynolds, Darin Madson, Mary Thurn, Paulo Fioravante, Albert Rovira, Rob McGaughey, Jamie Retallick, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings, Dennis Summers, Kenitra Hendrix, James Lyons, Joseph Boyle, Leticia Linhares, Edson Magalhães, Christopher Rademacher, Daniel Linhares, Marcelo Almeida, Giovani Trevisan, Zhong Peng   +54 more
wiley   +1 more source