Results 41 to 50 of about 2,919,643 (273)

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

Molecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez, Rafael Pérez‐Díaz, Hugo Ramos‐Solano, Marina Garrido‐Casado, Vanessa C. Talayero, Miguel Vicente‐Manzanares   +5 more
wiley   +1 more source

Delineating rockmass damage zones in blasting from in-field seismic velocity and peak particle velocity measurement

International Journal of Engineering, Science and Technology, 2011
Damage to the surrounding rock during blasting either in underground or surface excavation, is a growing concern today. It has been found that the integrity of rockmass is reduced significantly from pre to post blast condition due to disregard paid to the surrounding rockmass.
Dey, K, Murthy, VMSR
openaire   +3 more sources

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Monitoring of train-induced vibrations on rock slopes

International Journal of Distributed Sensor Networks, 2017
Train-induced vibrations will undoubtedly influence the stability of slopes near railway lines. To monitor the effects of such vibrations on slope stability, a wirelessly networked vibration test system was established, which included wirelessly ...
Jiangbo Xu, Changgen Yan, Xu Zhao, Ke Du, Heng Li, Yongli Xie   +5 more
doaj   +1 more source

Effective Assessment of Blast-Induced Ground Vibration Using an Optimized Random Forest Model Based on a Harris Hawks Optimization Algorithm

Applied Sciences, 2020
Most mines choose the drilling and blasting method which has the characteristics of being a cheap and efficient method to fragment rock mass, but blast-induced ground vibration damages the surrounding rock mass and structure and is a drawback. To predict,
Zhi Yu, Xiuzhi Shi, Jian Zhou, Xin Chen, Xianyang Qiu   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Rebound Characteristics of Wet-Shotcrete Particle Flow Jet from Wall Based on CFD-DEM

Buildings
This paper aims to reveal the motion law and collision behaviors of shotcrete particle flow jets. A physical model of the jet flow field composed of a nozzle structure and jet area was constructed and meshes with various sizes were used to mesh the ...
Lianjun Chen, Yang Zhang, Pengcheng Li, Gang Pan   +3 more
doaj   +1 more source

Experimental study on mesoscopic damage and fragmentation characteristics of hard rock under repeated particle impacts

Rock and Soil Mechanics, 2022
Particle impact, a new drilling technology, has been applied in drilling and gas and oil exploitation. Also, it is considerably promising as an assisting rock-breaking method for the excavation of tunnels in extremely hard rocks.
JU Ming-he, TAO Ze-jun, LI Xiao-feng, YU Li-yuan, JIANG Li-jie, LI Xiao-zhao   +5 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source