Results 141 to 150 of about 6,645 (176)

Efficient Over-expression and Application of High-performance Pectin Lyase by Screening Aspergillus niger Pectin Lyase Gene Family

Biotechnology and Bioprocess Engineering, 2018
Acidic pectin lyase is of great application value in fruit-processing industry. In this study, five pectin lyase genes (pelA, pelB, pelC, pelD and pelF) from A. niger pectin lyase gene family were cloned and over-expressed in A. niger strain SH-2, respectively.
Yulan He, Li Pan, Bin Wang
openaire   +1 more source

Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase

2005
Pectic substances are polysaccharides rich in galacturonic acid. They are particularly abundant in the middle lamellae of plant tissues where they hold leaf cells together. During decomposition of leaf litter in streams, fungal activity softens leaf tissue and may release individual cells (maceration).
openaire   +1 more source

[107] Pectin lyase from fungi

1966
Publisher Summary This chapter discusses the synthesis of pectin lyase from fungi. The method of choice is based on the rate of formation of ∆4:5-unsaturated uronide product. Conjugation of the double bond with the C-6 carbonyl results in absorbance of ultraviolet light maximally at 235 mμ.
openaire   +1 more source

[Demonstration of pectin-lyase in Bacillus subtilis].

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 1986
After a screening performed on pectinolytic micro-organisms, a strain of B. subtilis was isolated. This strain has a pectic enzyme capable of beta-elimination on highly methylated pectin (degree of esterification = 85%), without the action of a pectinesterase. This activity corresponds to that of a pectin-lyase.
A, Jauneau, O, Morvan, C, Morvan, M, Demarty, G, Devauchelle   +4 more
openaire   +1 more source

Application of mass spectrometry to determine the activity and specificity of pectin lyase A

Carbohydrate Research, 2002
Electrospray ionization (ESI) with quadrupole ion-trap mass spectrometry was used to assess the activity and specificity of the enzyme pectin lyase A (PLA) (EC 4.2.2.10) on model pectins with varying degrees and patterns of methyl esterification. PLA is a pectinase which cleaves alpha-(1-->4)-glycosidic linkages in pectin by a trans-elimination process.
Mutenda, K.E., Körner, R., Christensen, T.M.I.E., Mikkelsen, J., Roepstorff, P.   +4 more
openaire   +3 more sources

β-Transelimination of citrus pectin catalyzed by penicillium italicum pectin lyase in a membrane reactor

Applied Biochemistry and Biotechnology, 1995
Continuous β-transelimination of citrus pectin was achieved using pectin lyase [PNL, poly(methoxygalacturonide)lyase, EC 4.2.2.10] purified fromPenicillium italicum and confined in a membrane reactor. Various operational parameters, such as enzyme and substrate concentrations, filtrate flowrate, and reaction volume were optimized.
Itziar Alkorta, Carlos Garbisu, María J. Llama, Juan L. Serra   +3 more
openaire   +1 more source

Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides

Science China Life Sciences
Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity. Herein, we identified a polysaccharide lyase 1 protein, PsPL1, which acts as an essential virulence factor of P. sojae infection in soybean. However, the overexpression of PsPL1 in P.
Guangzheng, Sun, Yeqiang, Xia, Kuikui, Li, Qinsheng, Zhu, Feifei, Ding, Hui, Gu, Zhichao, Zhang, Xinrui, Li, Xuan, Mi, Jun, Chen, Ruoting, Yao, Sicong, Zhang, Haibing, Ouyang, Xi, Chen, Tengfei, Liu, Haibin, Jiang, Yao, Zhao, Min, Qiu, Wenwu, Ye, Kaixuan, Duan, Zhenchuan, Ma, Suomeng, Dong, Heng, Yin, Yan, Wang, Yuanchao, Wang   +24 more
openaire   +2 more sources

Enzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.

Journal of AOAC International, 2002
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of
K M, Hansen, A B, Truesen, J R, Søderberg   +2 more
openaire   +1 more source

Recent insights into microbial pectin lyases: A review

Process Biochemistry, 2023
Kanchan Yadav, Shruti Dwivedi, Supriya Gupta, Aiman Tanveer, Sangeeta Yadav, Pramod K. Yadav, Gautam Anand, Dinesh Yadav   +7 more
openaire   +1 more source

Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae

Journal of Hazardous Materials, 2021
Chaojun Zhang, Canming Tang
exaly