Results 261 to 270 of about 50,025 (309)
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A reactive aspartyl residue of pepsin
Biochemical and Biophysical Research Communications, 1968Abstract Recently it was reported ( Hamilton, Spona, and Crowell, 1967 ) that pepsin was inactivated by an equimolar amount of 1-diazo-4-phenylbutanone-2 (DPB). The results indicated that the reaction occurred at or near the active site of pepsin. In the present communication we report evidence indicating that DPB reacts with pepsin to form an ester ...
K T, Fry +4 more
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Pepsins—A chromatographic and kinetic comparison
Analytical Biochemistry, 1969Abstract A simplified procedure for the preparation of pepsin from porcine pepsinogen has been developed. Chromatographic and kinetic data indicate that this procedure yields a pepsin product identical with that obtained by the conventional method.
R, Trujillo, M, Schlamowitz
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Bovine pepsinogen and pepsin. IV. A new method of purification of the pepsin
Biochimica et Biophysica Acta (BBA) - Enzymology, 1971A new purification procedure for bovine pepsin (EC 3.4.4.1) based on affinity chromatography, is more rapid, and gives a higher yield and activity than previous procedures. It may be used for purification of other pepsins and of pepsinogen. Pepsinogen is extracted from gastric mucosa, fractionated with ammonium sulfate, and activated to pepsin.
B, Nevaldine, B, Kassell
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Pepsin as a Marker of Extraesophageal Reflux
Annals of Otology, Rhinology & Laryngology, 2010Diagnosis of extraesophageal reflux (EER) currently relies on tools designed for diagnosis of gastroesophageal reflux. Such tools lack the sensitivity and reproducibility to detect the less frequent and mildly acidic reflux associated with upper airway disease. Pepsin has been posited to be a reliable biological marker of EER. Our aim was to present a
Tina L, Samuels, Nikki, Johnston
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A useful spectrophotometric rate assay for pepsin
Analytical Biochemistry, 1971Abstract Phenyl sulfite is a specific substrate for pepsin, whose hydrolysis can be followed spectrophotometrically. This compound is superior to peptide substrates in its ease of synthesis and its ability to be monitered spectrophotometrically. The sulfite assay is therefore the method of choice for both routine and detailed assays of pepsin.
T P, Stein, T W, Reid, D, Fahrney
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Pepsin as a Marker for Pulmonary Aspiration
American Journal of Critical Care, 2002• Background Although assessment for aspiration of small volumes of gastric contents in tube-fed patients receiving mechanical ventilation is important, available methods for this purpose are not wholly satisfactory. A potential method is immunoassay of tracheal secretions for the gastric enzyme pepsin.
Norma A, Metheny +8 more
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Chicken Pepsin as a Rennet Substitute
Canadian Institute of Food Science and Technology Journal, 1984Abstract Semi-purified chicken pepsin was prepared from proventriculae at a yield of 33% of the original pepsin activity using a procedure based on the method described by Bohak (1970) . Cheddar cheese was produced using the chicken pepsin preparation, and with calf rennet as a control, in two trials.
C.J. Findlay, D.W. Stanley, D.B. Emmons
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Pepsin and antacid therapy: A dilemma
Journal of Chemical Education, 1979Illustrates some areas of uncertainty in the current literature regarding the mechanism of digestion and presents an experiment that is more consistent with current biochemical research.
Patty H. Laswick, W. Brayton Batson
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A History Of Pepsin And Related Enzymes
The Quarterly Review of Biology, 2002Studies on gastric digestion during 1820-1840 led to the discovery of pepsin as the agent which, in the presence of stomach acid, causes the dissolution of nutrients such as meat or coagulated egg white. Soon afterward it was shown that these protein nutrients were cleaved by pepsin to diffusible products named peptones.
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Gastrin, a Stimulant of Pepsin Secretion
Archives of Surgery, 1963In theoretical considerations of the mechanism of gastric secretion, suggestions have been made that irrespective of whether the initial stimulus to the gastric glands is nervous by way of the vagi or hormonal by way of gastrin, the ultimate final common pathway for these stimuli may be the release of histamine.
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