Results 261 to 270 of about 40,308 (299)

Rapid colorimetric assay for intestinal peptide hydrolases

Analytical Biochemistry, 1974
Abstract A simple two-step method is described for quantitating the release of free l -phenylalanine, l -leucine, l -methionine, or l -isoleucine from di- or polypeptides. The colorimetric assay is based on the ability of l -amino acid oxidase to catalyze the oxidation of free l -amino acid, but not of peptides.
C R, Shoaf, K J, Isselbacher, W D, Heizer   +2 more
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A high-molecular weight peptide hydrolase in erythrocytes

International Journal of Biochemistry, 1982
1. A peptide hydrolase has been partially purified from the soluble fraction of erythrocyte lysates. 2. The enzyme has a molecular weight of approximately 600,000 and hydrolyses the chymotrypsin substrate glutaryl-Gly-Gly-Phe-7-amido-4-methylcoumarin (pH optimum 7.0) and the trypsin substrate CBZ-Gly-Gly-Arg-2-naphthylamide.
T, Edmunds, R J, Pennington
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Peptide hydrolases in spinal cord and brain of the rabbit

Brain Research, 1972
Summary Spinal cord and brain fractions from rabbits were compared in terms of their content of free amino acids, the amino acid composition of their proteins, and the level of selected proteases and peptidases. The amino acid composition of poteins in the cervical, thoracic and lumbar areas of the cord were similar, and no major differences were ...
S, Serra, A, Grynbaum, A, Lajtha, N, Marks   +3 more
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A Study of Intraluminal Peptide Hydrolase Activity in the Rat

Clinical Science, 1975
1. Uncentrifuged and centrifuged rat intestinal contents were assayed for peptide hydrolase activity with glycyl-l-phenylalanine (Gly-Phe) and l-phenylalanyl-glycine (Phe-Gly) as substrates in the absence and presence of the intestinal cytosol peptide hydrolase inhibitor p-hydroxymercuribenzoate. 2.
D B, Silk, Y S, Kim
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PROTEIN AND PEPTIDE HYDROLASES OF THE RAT HYPOTHALAMUS AND PITUITARY

Journal of Neurochemistry, 1974
AbstractPeptide and peptidyl‐peptide hydrolase activities were measured in the cerebral cortex, adenoand neurohypophysis, anterior and posterior regions of the hypothalamus to assess regional differences in peptide hormone turnover. In general all enzyme activities were higher in the pituitary except for the monoacyl arylamidase which was lower, and ...
N, Marks, A, Galoyan, A, Grynbaum, A, Lajtha   +3 more
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Peptide‐Based Catalyst Mimicking Hydrolase Enzyme

Journal of Peptide Science
ABSTRACTPeptide‐based nanomolecular constructs offer great possibilities for designing catalytic molecular systems mimicking enzymes. In this study, we designed three tripeptide catalysts that can possibly mimic hydrolase enzymes, with the objective of systematically verifying the scope of modulating enzymatic activity.
Kalpana Kumari, Vivek Prakash, Naveen Kumar, Vibin Ramakrishnan   +3 more
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Peptide hydrolases in holothurian intestinal mucosa

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1981
Abstract 1. Peptide hydrolases from intestinal mucosa of H. forskali and N. mixta were studied by starch gel electrophoresis. 2. Dipeptides hydrolyzed were L -Leu- L -Leu and L -Gly- L -Leu. L-Gly- L -Tyr and L -Leu- L -Tyr were not 3. L -Leu- L -Leu- L -Leu-was hydrolyzed by extracts of both species. 4.
S. McGettigan, M. Canning, G. O'Cuinn, D.B. Johnson   +3 more
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Artificial hydrolase based on carbon nanotubes conjugated with peptides

Nanoscale, 2016
An artificial enzyme was constructed by attaching short peptides with active sites (SHELKLKLKL, WLKLKLKL) onto carbon nanotubes (CNT). It was found that the combination of SHE amino acids was essential to form a catalytic triad. W was also incorporated into this artificial enzyme and acted as a substrate binding site, thus producing an enzyme model ...
Qiang, Zhang, Xingxing, He, Ailing, Han, Qingxia, Tu, Guozhen, Fang, Jifeng, Liu, Shuo, Wang, Haibo, Li   +7 more
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Enzyme‐mediated peptide synthesis using acylpeptide hydrolase

European Journal of Biochemistry, 1991
Acylpeptide hydrolase is shown to catalyse the specific addition of a single amino acid to the N‐terminus of a peptide. The stabilised Sepharose‐coupled form of the enzyme is used to couple a carboxy‐methylated N‐formyl (or N‐acetyl) amino acid to a short pre‐existing peptide.
T C, Farries, A D, Auffret, A, Aitken
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INHIBITION AND ACTIVATION OF BARLEY PEPTIDE HYDROLASES II. PEPTIDE HYDROLASES B AND C, AND L-LEUCYL-β-NAPHTHYLAMIDASE

Journal of the Institute of Brewing, 1971
Peptide hydrolase B is inhibited by DIPF and appears to be a “serine esterase” enzyme. Para-chloromercuribenzoate is also inhibitory, but it acts irreversibly and not as a mercaptide former. Dithiothreitol acts irreversibly to inhibit the enzyme and thioglycolate is a weak, reversible inhibitor. Peptide hydrolase C is also inhibited by DIPF, and
W. C. Burger, Neville Prentice, Mary Moeller   +2 more
openaire   +1 more source