Results 121 to 130 of about 10,311,340 (298)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparative study of aeroacoustic performance of 1/8 and 1/1 pantographs coupled with cavity

Railway Engineering Science
The technology of pantograph sinking in the cavity is generally adopted in the new generation of high-speed trains in China for aerodynamic noise reduction in this region. This study takes a high-speed train with a 4-car formation and a pantograph as the
Xiaoming Tan, Huifang Liu, Zhigang Yang, Hong Chen, Baojun Fu, Linli Gong   +5 more
doaj   +1 more source

Work of Breathing for Aviators: A Missing Link in Human Performance

Life
In this study, we explore the work of breathing (WoB) experienced by aviators during the Anti-G Straining Maneuver (AGSM) to improve pilot safety and performance. Traditional airflow models of WoB fail to adequately distinguish between breathing rate and
Victoria Ribeiro Rodrigues, Rheagan A. Pratt, Chad L. Stephens, David J. Alexander, Nicholas J. Napoli   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A performance-based optimization method for topology design of continuum structures with mean compliance constraints

, 2002
[Abstract]: A performance-based optimization (PBO) method for optimal topology design of linear elastic continuum structures with mean compliance constraints is presented in this paper.
Steven, Grant P., Liang, Qing Quan
core   +1 more source

Intensity Thresholds for External Workload Demands in Basketball: Is Individualization Based on Playing Positions Necessary?

Sensors
Currently, basketball teams use inertial devices for monitoring external and internal workload demands during training and competitions. However, the intensity thresholds preset by device manufacturers are generic and not adapted for specific sports (e.g.
Sergio J. Ibáñez, Carlos D. Gómez-Carmona, Pablo López-Sierra, Sebastián Feu   +3 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Short-Term Effects of Plyometric Training and Subsequent Detraining on Sprint Performance, Change-of-Direction Ability and Muscle Size-Related Changes in Prepubertal Boys

Applied Sciences
Background: Plyometric training is widely used to enhance explosive performance in youth athletes, yet limited evidence exists regarding the persistence of neuromuscular and morphological adaptations after training cessation in prepubertal populations ...
Majdi Dridi, Roland van den Tillaar, Abdelkader Mahmoudi, Haithem Rebai, Raouf Hammami   +4 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Progressive tendon-loading eccentric exercise therapy in athletes with patellar tendinopathy improves postural control, quadriceps strength, and pain: A randomized clinical trialWhat are the new findings?

Journal of ISAKOS
Introduction/objectives: Athletes with patellar tendinopathy (PT) have impaired postural control and an increased subsequent sport injury risk of sports injuries. Improving their postural control is crucial to reduce future sports injuries.
Thouraya Fendri, Bruno Beaune, Haithem Rebai, Fatma Chaari, Mohammed Achraf Harrabi, Abderrahmane Rahmani, Sébastien Boyas, Sonia Sahli   +7 more
doaj   +1 more source