Results 331 to 340 of about 412,823 (364)

Proliferation of Peripheral Blood Mononuclear Cells Increases Riboflavin Influx

Proceedings of the Society for Experimental Biology and Medicine, 2000
Abstract. Previously we demonstrated that proliferation of peripheral blood mononuclear cells (PBMC) causes a five‐fold increase in cellular uptake of biotin; this increase is mediated by an increased number of biotin transporters on the PBMC surface.
J, Zempleni, D M, Mock
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Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood

Current Protocols in Immunology, 2009
AbstractPeripheral blood is the primary source of lymphoid cells for investigation of the human immune system. Its use is facilitated by Ficoll‐Hypaque density gradient centrifugation—a simple and rapid method of purifying peripheral blood mononuclear cells (PBMC) that takes advantage of the density differences between mononuclear cells and other ...
Fuss, I., Kanof, M., Smith, P., Zola, H.
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Esterase staining of human peripheral blood mononuclear cells

Journal of Immunological Methods, 1979
Recently proposed esterase staining methods for the cytochemical identification of human peripheral blood monocytes and lymphocytes in our hands gave suboptimal results. Cellular purification and recommended fixation procedures appeared to decrease the esterase reactivity of leucocyte preparations.
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Generation of neuronal cells from human peripheral blood mononuclear cells

NeuroReport, 2010
We have examined the potency of two methods for the neuronal differentiation of embryonic stem cells on the generation of neuronal cells from human blood cells. A mixture of mononuclear cells from peripheral blood cells expressing monocytic, hematopoietic, and mesenchymal cell surface markers were exposed to all-trans retinoic acid, epidermal growth ...
Sandra, Horschitz, Andreas, Meyer-Lindenberg, Patrick, Schloss   +2 more
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HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells

2014
Human immunodeficiency virus 1 (HIV-1) isolation from peripheral blood mononuclear cells (PBMCs) allows retrieval of replication-competent viral variants. In order to impose the smallest possible selective pressure on the viral isolates, isolation must be carried out in primary cultures of cells and not in tumor derived cell lines.
Dispinseri, Stefania, Saba, Elisa, Vicenzi, Elisa, Kootstra, Neeltje A., Schuitemaker, Hanneke, Scarlatti, Gabriella   +5 more
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Cryopreservation of Peripheral Blood Mononuclear Cells

2016
The use of cryopreserved peripheral blood mononuclear cells (PBMC) for the studies of immune reconstitution in human immunodeficiency virus (HIV)-infected patients permits the selection of samples from well-characterized study subjects. The goal of the cryopreservation is to freeze and thaw the PBMC without compromising cell viability.
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Jacalin Regulates IgA Production by Peripheral Blood Mononuclear Cells

Immunotherapy, 2012
In IgA nephropathy, circulating immune complexes containing IgA1 are deposited on the glomerular mesangium, causing mesangial cell proliferation and acceleration of extracellular matrix production. The suppressive effect of jacalin, a galactose-binding lectin, on IgA production in vitro was determined.Normal human peripheral blood mononuclear cells ...
Keiichi, Miyamoto, Takeshi, Chiba, Noriko, Shinohara, Yuko, Nagata, Nagisa, Asakawa, Shinji, Kato, Naoki, Mizutani, Tomohiro, Murata, Shinsuke, Nomura, Takashi, Horiuchi   +9 more
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Peripheral Blood Mononuclear Cells

2023
Sara Carella, Maria Giuseppina Onesti
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Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

2014
The work with peripheral blood mononuclear cells (PBMCs), which comprise lymphocytes and monocytes, is indispensable in immunological diagnostics and research. The isolation of PBMCs takes advantage of differences in cell density of the different blood components.
Christine, Riedhammer, Dagmar, Halbritter, Robert, Weissert   +2 more
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Peripheral Blood Mononuclear Cells for Limb Ischemia

2017
There is accumulating evidence that the peripheral blood is a source of pro-angiogenic mononuclear cells (MNCs). These cells were initially described as endothelial progenitor cells (EPCs) expressing CD34 and vascular endothelial growth factor (VEGF) receptor 2.
Masayoshi Suda, Ippei Shimizu, Yohko Yoshida, Tohru Minamino   +3 more
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