Results 51 to 60 of about 150,436 (290)

Gene Electrotransfer Efficiency in 2D and 3D Cancer Cell Models Using Different Electroporation Protocols: A Comparative Study

Pharmaceutics, 2023
Electroporation, a method relying on a pulsed electric field to induce transient cell membrane permeabilization, can be used as a non-viral method to transfer genes in vitro and in vivo.
Alexia de Caro, Elisabeth Bellard, Jelena Kolosnjaj-Tabi, Muriel Golzio, Marie-Pierre Rols   +4 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Mitochondrial respiration in human peripheral blood mononuclear cells: methodology and influence of permeabilization and storage

Frontiers in Molecular Biosciences
Human peripheral blood mononuclear cells (PBMCs) can be easily sampled from healthy individuals and patients. Density gradient isolation from human blood or leukocyte concentrates yields a mononuclear cell population of mainly lymphocytes, monocytes, and
Shusuke Sekine, Shusuke Sekine, Imen Chamkha, Evelina Elmér, Evelina Elmér, Eleonor Åsander Frostner, Emil Westerlund, Emil Westerlund, Tianshi Liu, Johannes Ehinger, Johannes Ehinger, Fredrik Sjövall, Fredrik Sjövall, Hiroyuki Uchino, Eskil Elmér, Eskil Elmér   +15 more
doaj   +1 more source

Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells

FEBS Letters, EarlyView.
DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park, Inho Kwon, Yong Hwan Kim, Ji‐Eun Bae, Joon Bum Kim, Seong Hyun Kim, Sungho Maeng, Doo Sin Jo, Dong‐Hyung Cho   +8 more
wiley   +1 more source

Membrane permeabilization assay of salivaricin 9.

, 2013
A: Salivaricin 9 permeabilization activity towards cytoplasmic membrane of S. equisimilis. B: Salivaricin 9 permeabilization activity towards cytoplasmic membrane of Corynebacterium spp.
Abdelahhad Barbour (471696), Sekaran Muniandy (134168), Koshy Philip (471697)   +2 more
core   +1 more source

Caspase-8 deficiency induces a switch from TLR3 induced apoptosis to lysosomal cell death in neuroblastoma

Scientific Reports, 2021
In cancer cells only, TLR3 acquires death receptor properties by efficiently triggering the extrinsic pathway of apoptosis with Caspase-8 as apical protease.
Marie-Anaïs Locquet, Gabriel Ichim, Joseph Bisaccia, Aurelie Dutour, Serge Lebecque, Marie Castets, Kathrin Weber   +6 more
doaj   +1 more source

Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers

Sensors, 2022
Membrane permeabilization stimulated by high-voltage electric pulses has been used to deliver cell-impermeable exogenous molecules. The electric field effect on the cells depends on various experimental parameters, such as electric field strength, the ...
Yuki Watanabe, Hirohito Nihonyanagi, Rika Numano, Takayuki Shibata, Kazunori Takashima, Hirofumi Kurita   +5 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide: Melittin

The permeabilization of liposomes by melittin, an antimicrobial peptide (AMP), has been studied by an electrochemiluminescence (ECL) imaging strategy. The methodology consisted first of encapsulating ECL reagents in sealed giant asymmetrical liposomes ...
Jérôme Delacotte (8841911), Eric Labbé (1893355), Frédéric Lemaître (2380273), Manon Guille-Collignon (11919882), Stéphane Arbault (2324968), Fatma Ben Trad (11919876), Neso Sojic (1460824), Fabienne Burlina (24089), Olivier Buriez (2356657)   +8 more
core   +3 more sources

Compact Electro-Permeabilization System for Controlled Treatment of Biological Cells and Cell Medium Conductivity Change Measurement

Measurement Science Review, 2014
Subjection of biological cells to high intensity pulsed electric field results in the permeabilization of the cell membrane. Measurement of the electrical conductivity change allows an analysis of the dynamics of the process, determination of the ...
Novickij Vitalij, Grainys Audrius, Novickij Jurij, Tolvaisiene Sonata, Markovskaja Svetlana   +4 more
doaj   +1 more source