Results 211 to 220 of about 4,214 (236)

Assessing Pexophagy in Mammalian Cells

2017
In mammalian cells several hundred peroxisomes are maintained by a balance between the biogenesis and turnover by peroxisome homeostasis. Pexophagy, a form of autophagy specific for peroxisomes, is the main pathway for peroxisome degradation, but molecular mechanisms of mammalian pexophagy are largely unknown.
Shun-Ichi, Yamashita, Yukio, Fujiki
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Catalase inhibition induces pexophagy through ROS accumulation

Biochemical and Biophysical Research Communications, 2018
Peroxisomes are dynamic and multifunctional organelles involved in various cellular metabolic processes, and their numbers are tightly regulated by pexophagy, a selective degradation of peroxisomes through autophagy to maintain peroxisome homeostasis in cells.
Joon No, Lee, Raghbendra Kumar, Dutta, Yunash, Maharjan, Zhi-Qiang, Liu, Jae-Young, Lim, Se-Jin, Kim, Dong-Hyung, Cho, Hong-Seob, So, Seong-Kyu, Choe, Raekil, Park   +9 more
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Experimental Systems to Study Yeast Pexophagy

2017
Peroxisome abundance is tightly regulated according to the physiological contexts, through regulations of both proliferation and degradation of the organelles. Here, we describe detailed methods to analyze processes for autophagic degradation of peroxisomes, termed pexophagy, in yeast organisms.
Shun-Ichi, Yamashita, Masahide, Oku, Yasuyoshi, Sakai, Yukio, Fujiki   +3 more
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Pexophagy: Autophagy of Peroxisomes

2010
The autophagic removal of peroxisomes is called pexophagy. Pexophagy goes on at a basal level. It also results in the removal of excess peroxisomes induced by experimental manipulation of rats as illustrated and discussed here.
Margit Pavelka, Jürgen Roth
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Pexophagy in Hansenula polymorpha.

Methods in enzymology, 2008
In the yeast Hansenula polymorpha the development and turnover of peroxisomes is readily achieved by manipulation of the cultivation conditions. The organelles massively develop when the cells are incubated in the presence of methanol as the sole source of carbon and energy.
Zutphen, Tim van, Klei, Ida J. van der, Kiel, Jan A.K.W.   +2 more
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Fluorescence Methods to Measure Pexophagy

We outline our approach for studying the selective autophagy of peroxisomes (pexophagy), using fluorescence microscopy in tissue cell culture models. Ratiometric reporters, which specifically localize to peroxisomes, allow a quantitative assessment of pexophagy in fixed and live cells, as well as whole organisms.
Francesco G, Barone, Sylvie, Urbé, Michael J, Clague   +2 more
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Molecular Machines Involved in Pexophagy

2014
Pexophagy, the selective autophagy of peroxisomes, is a membrane trafficking pathway that delivers damaged or superfluous peroxisomes from the cytosol to the vacuole/lysosome for degradation and recycling. Pexophagosomes, the peroxisome-containing autophagosomes, are the double-membrane carriers in the pexophagy pathway.
Taras Y. Nazarko, Jean-Claude Farré
openaire   +1 more source

Pexophagy in Pichia pastoris.

Methods in enzymology, 2009
The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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Mechanisms of autophagy and pexophagy in yeasts

Biochemistry (Moscow), 2011
Autophagy is a process of recycling of the intracellular constituents using vacuoles (lysosomes). General autophagy occurs due to involvement of highly conservative components found in all eukaryotes, from yeasts to higher plants and humans. Autophagy also could be a selective process and be involved in regulation of the cellular number of organelles ...
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Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration

2007
Pichia pastoris has proven to be a valuable model for examining the molecular events of the selective degradation of peroxisomes by a process called pexophagy. We have developed a protocol to rapidly identify genes essential for glucose-induced pexophagy.
Laura A, Schroder, Benjamin S, Glick, William A, Dunn   +2 more
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